干旱区生态环境知识资源中心(Arid): 液相色谱-串联质谱法检测贝类产品中腹泻性贝类毒素

Arid

	液相色谱-串联质谱法检测贝类产品中腹泻性贝类毒素
其他题名	Determination of Diarrhetic Shellfish Poisoning in Shellfishes by Liquid Chromatography with Tandem Mass Spectrometry
	母清林 1; 方杰 1; 万汉兴 2; 王晓华 1; 曹柳燕 1; 张庆红 1
来源期刊	分析化学
ISSN	0253-3820
出版年	2011
卷号	39 期号:1 页码:111-114
中文摘要	建立了贝类组织中2种腹泻性贝毒(Diarrhetic Shellfish Poisoning,DSP)聚醚类毒素-大田软海绵酸(Okadaic acid,OA)和鳍藻毒素(Dinophysistoxin-1,DTX-1)的高效液相色谱-串联质谱分析方法.贝类样品经80%甲醇溶液提取,经正己烷脱脂和HLB固相萃取柱净化,采用Pursuit C18(150mm%3.0mm,3mum, Varian公司)色谱柱分离,以80%(V/V)甲醇水溶液为流动相等度洗脱,负离子扫描,在多反应监测(MRM)模式下进行定性与定量分析.在4~200mug/L范围内线性关系良好,OA和DTX-1的最低定量限均为2mug/kg.添加水平在2,25和50mug/kg时的平均回收率大于80%,相对标准偏差小于11%(n=6)
英文摘要	A liquid chromatography-electrospray ionization triple-quadruple tandem mass spectrometric method has been established for the determination of two kinds of diarrhetic shellfish poisoning including okadaic acid(QA) and dinophysistoxin-1(DTX-1). After being extracted using methanol and water(80:20,V/V), the solution was extracted with n-hexane to remove lipid components and then cleaned-up by solid phase extraction(SPE) on an Oasis HLB cartridge. The analytes were eluted with methanol-water(80:20,V/V) on a Pursuit C18 column(150 mm * 3.0 mm,3 mum).The quantitative and confirmatory determination of OA and DTX-1 was performed by multiple reactions monitoring(MRM) mode. OA and DTX-1 were determined in the negative ion mode. The calibration curve was linear in the range of 4-200mug/L.The quantification limits of OA and DTX-1 were 2mug/kg. The mean recoveries at spiked concentration of 2-100 mug/kg were more than 80% and the relative standard deviations were less than 11%(n = 6)
中文关键词	液相色谱-串联质谱 ; 贝类毒素 ; 大田软海绵酸 ; 鳍藻毒素 ; 贝类产品
英文关键词	Liquid chromatography tandem mass spectrometry Shellfish poisoning Okadaic acid Dinophysistoxin-1 Shellfish
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENGINEERING CHEMICAL
WOS研究方向	Engineering
CSCD记录号	CSCD:4114650
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226373
作者单位	1.浙江省舟山海洋生态环境监测站, 舟山, 浙江 316000, 中国; 2.中国海洋大学环境科学与工程学院, 青岛, 山东 266100, 中国
推荐引用方式 GB/T 7714	母清林,方杰,万汉兴,等. 液相色谱-串联质谱法检测贝类产品中腹泻性贝类毒素[J],2011,39(1):111-114.
APA	母清林,方杰,万汉兴,王晓华,曹柳燕,&张庆红.(2011).液相色谱-串联质谱法检测贝类产品中腹泻性贝类毒素.分析化学,39(1),111-114.
MLA	母清林,et al."液相色谱-串联质谱法检测贝类产品中腹泻性贝类毒素".分析化学 39.1(2011):111-114.