干旱区生态环境知识资源中心(Arid): 液相色谱-串联质谱法测定水稻中生长素及其氨基酸结合物

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	液相色谱-串联质谱法测定水稻中生长素及其氨基酸结合物
其他题名	Analysis of Auxins and Their Amino Acid Conjugates in Rice by Liquid Chromatography Tandem Mass Spectrometry
	曹赵云 1; 马有宁 2; 陈铭学 2; 牟仁祥 2; 胡秀芳 1
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2011
卷号	30 期号:12 页码:1419-1424
中文摘要	建立了同时测定水稻中吲哚-3-丁酸(IBA)、吲哚-3-乙酸(IAA)及其7种氨基酸结合物的液相色谱-串联质谱（HPLC-MS/MS)检测方法。样品在4℃下于80%甲醇中浸提12 h后，经混合阴离子交换反相固相萃取(MAX)净化，以5 mmol/L的甲酸铵溶液和甲醇为流动相，在C_18柱上进行液相色谱分离，电喷雾正离子模式下用三重四极杆质谱仪多反应监测方式测定。对水稻根、茎、叶和籽粒4种基质分别进行加标回收实验，9种分析物的平均加标回收率为75%~106%,USD为3.6%~15.2%，方法检出限（S/N = 3)为0.01~25 mug/kg。方法准确、灵敏，能满足水稻中9种生长素及其结合物在其生理水平的测定。
英文摘要	A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed for the simultaneous determination of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and its 7 amino acid conjugates including IAA-Asp, IAA-Ala, IAA-Val, IAA-Trp, IAA-Leu, IAA-lie and IAA-Phe in rice. The sample was extracted with 80% methanol for 12 h at 4 °C. The extract was cleaned up on an Oasis MAX cartridge(60 mg * 3 mL), and eluted with methanol contained 0. 5% formic acid. 9 analytes were separated on a C_18 column (1.8 mum, 100 mm * 2.1 mm i. d.) using 5 mmol/L ammonium formate (A)-acetonitrile (B) as mobile phase by gradient elution. The analytes were detected by positive-ion electrospray ionization-mass spectrometry under multiple reaction monitoring(MRM) mode. The recoveries of 9 analytes from the matrixes of root, sheath, leaf and seed in rice tissue ranged from 75% to 106% with relative standard deviations(RSDs) of 3.6%-15.2%. The detection limits(S/N = 3) ranged from 0.01 mug/kg to 25 mug/kg. The method was sensitive and reliable, and could meet the requirements for quantification of auxins and their amino acid conjugates in rice at the physiological level.
中文关键词	液相色谱-串联质谱 ; 生长素 ; 氨基酸结合物 ; 水稻
英文关键词	liquid chromatography-tandem mass spectrometry(LC-MS/MS) auxin amino acid conjugates rice
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4401796
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226372
作者单位	1.浙江理工大学生命科学学院, 杭州, 浙江 310018, 中国; 2.中国水稻研究所农业部稻米及制品质量监督检验测试中心, 杭州, 浙江 310006, 中国
推荐引用方式 GB/T 7714	曹赵云,马有宁,陈铭学,等. 液相色谱-串联质谱法测定水稻中生长素及其氨基酸结合物[J],2011,30(12):1419-1424.
APA	曹赵云,马有宁,陈铭学,牟仁祥,&胡秀芳.(2011).液相色谱-串联质谱法测定水稻中生长素及其氨基酸结合物.分析测试学报,30(12),1419-1424.
MLA	曹赵云,et al."液相色谱-串联质谱法测定水稻中生长素及其氨基酸结合物".分析测试学报 30.12(2011):1419-1424.