干旱区生态环境知识资源中心(Arid): 果汁中天然色素紫胶红的UPLC/TUV定量检测及UPLC-MS/MS结构确证

Arid

	果汁中天然色素紫胶红的UPLC/TUV定量检测及UPLC-MS/MS结构确证
其他题名	Determination and Identification of Natural Pigment of Laccaic Acids in Juice by UPLC/TUV and UPLC-MS/MS
	梅梅; 杨闻翰; 杜振霞
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2011
卷号	30 期号:12 页码:1338-1344
中文摘要	建立了天然色素紫胶红各组分的UPLC/TUV定量检测及UPLC-MS/MS结构确证方法，并将所建立的分析方法应用于果汁中紫胶红色素的定性定量检测。通过Oasis@HLB小柱固相萃取技术净化、提取果汁饮料样品中的紫胶红色素。使用Waters Acquity UPLC BEH Shield RP C_(18)(1.7mum, 2.1 mm * 100 mm)色谱柱，以乙腈和0.1%甲酸水溶液为流动相梯度洗脱，经UPLC分离，TUV检测器在286 nm波长下，串联质谱SIR模式检测，并通过MS/MS断裂机理分析对天然色素紫胶红中的各组分进行结构确证。采用面积归一化法确定紫胶红A、紫胶红B、紫胶红C与紫胶红E的相对含量分别为57.95%/31.33%/6.12%/2.96%。采用外标法定量，4种混合组分在0.029?11 mg/L范围内线性良好（r > 0.999)，回收率为83%?111%,RSD为2.3%?9.2%，检出限为0.008 7?0.17 mg/kg，定量下限为0.029?0.58 mg/kg，可满足食品中天然色素含量的分析要求。
英文摘要	An ultra performance liquid chromatography-tandem mass spectrometric (UPLC/TUV-MS/MS) method was developed for the determination and validation of laccaic acids in juice. The juice samples were cleaned by solid-phase extraction technique with Oasis?HLB cartridge. The targeted compounds were separated on a Waters Acquity UPLC BEH Shield RP C_(18) column with aceton-trile-0.1% formic acid as mobile phase by gradient elution, and detected with TUV detector at 286 nm. A quadrupole mass spectrometer with atmospheric electrospray ionization source was used for the identification under SIR mode and the collision induced dissociation analysis. The external standard method was used for quantitation analysis. It was found that the lac dye extract consisted of laccaic A, laccaic B, laccaic C and laccaic E, of which the relative contents were 57.95%, 31.33%, 6.12% and 2.96%, respectively, based on the area normalization method, and the calibration curves for four laccaic acids showed good linearities in the range of 0.029-11 mg/L with correlation coefficients higher than 0.999. The average recoveries of high, medium and low three spiked concentration levels were between 83% and 111%, with relative standard deviations(RSDs) of 2.3%-9.2%. The limits of detection (LOD) were in the range of 0.008 7-0.17 mg/kg for the four components in juice matrix, and their limits of quantitation(LOQ) were 0.029-0.58 mg/kg. The results demonstrated that the proposed method for laccaic acids determination in juice could meet the requirements for the natural color analysis in food safety.
中文关键词	超高效液相色谱/可调谐紫外 ; 超高效液相色谱串联四极杆质谱 ; 天然色素 ; 紫胶红 ; 果汁饮料
英文关键词	UPLC/TUV UPLC-MS/MS natural pigment laccaic acids juice
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL ; FOOD SCIENCE TECHNOLOGY
WOS研究方向	Chemistry ; Food Science & Technology
CSCD记录号	CSCD:4401781
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226371
作者单位	北京化工大学理学院分析测试中心, 北京 100029, 中国
推荐引用方式 GB/T 7714	梅梅,杨闻翰,杜振霞. 果汁中天然色素紫胶红的UPLC/TUV定量检测及UPLC-MS/MS结构确证[J],2011,30(12):1338-1344.
APA	梅梅,杨闻翰,&杜振霞.(2011).果汁中天然色素紫胶红的UPLC/TUV定量检测及UPLC-MS/MS结构确证.分析测试学报,30(12),1338-1344.
MLA	梅梅,et al."果汁中天然色素紫胶红的UPLC/TUV定量检测及UPLC-MS/MS结构确证".分析测试学报 30.12(2011):1338-1344.