干旱区生态环境知识资源中心(Arid): UPLC - ESI MS/MS法测定动物饲料中苯二氮卓类药物

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	UPLC - ESI MS/MS法测定动物饲料中苯二氮卓类药物
其他题名	Determination of Benzodiazepines in Animal Feeds by UPLC-ESI MS/MS
	严丽娟 1; 张峰 2; 吴敏 1; 吴抒怀 1; 储晓刚 2; 周昱 1
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2011
卷号	30 期号:11 页码:1301-1305
中文摘要	建立了超高效液相色谱-电喷雾串联质谱(UPLC-ESIMS/MS)同时检测动物饲料中地西泮、奥沙西泮、硝西泮、三唑仑、艾司唑仑和咪达唑仑6种苯二氮卓类药物的方法。饲料样品采用碱性叔丁基甲醚提取，Oasis MCX固相萃取柱净化，反相色谱柱分离，以0.1%甲酸和乙腈为流动相进行梯度洗脱，正离子模式扫描，多反应监测模式检测，外标法定量。结果表明，6种苯二氮卓类药物在5~100 mug/L范围内呈良好线性，方法的检出限为2 mug/kg，定量下限为5 mug/kg。在空白大猪配合饲料中添加5、20、100 mug/kg 3个加标水平，6种药物的平均回收率为68%~94%，相对标准偏差为5.8%~12.7%。方法快速、准确、灵敏度高、重复性好，适用于动物饲料中苯二氮卓类药物的检测。
英文摘要	An ultra-performance liquid chromatography - electrospray ionization tandem mass spectrometric (UPLC - ESI MS/MS) method was developed for the simultaneous determination of six benzodiazepines, including diazepam, oxazepam, nitrazepam, triazolam, estazolam and midazoalm, in animal feeds. The benzodiazepines in the feeds were extracted with alkaline tert-butyl methyl ether followed by clean-up with Oasis MCX cartridge. The LC separation was performed on a Waters Acqu- ity UPLC BEH C_(18) column(50 mm * 2.1 mm, 1.7 mum) by gradient elution using 0.1% formic acid and acetonitrile as mobile phase at a flow rate of 0.3 mL/min. The identification of six drugs was achieved by electrospray ionization tandem mass spectrometer under multiple reaction monitoring (MRM) mode and the quantification analysis was performed by the external standard method. The calibration curves showed good linearities in the range of 5 - 100 mug/L. The limits of detection (LOD) for the six drugs in feeds were 2 mug/kg and the limits of quantitation (LOQ) were 5 mug/kg. The average recoveries of the six drugs at three spiked levels of 5, 20,100 mug/kg were between 68% and 94% with RSDs of 5.8% - 12.7%. The method was rapid, accurate and sensitive, and could be applicable in the routine assay on benzodiazepines in animal feeds.
中文关键词	苯二氮卓类 ; 超高效液相色谱-串联质谱法 ; 饲料
英文关键词	benzodiazepines ultra-performance liquid chromatography - tandem mass spectrometry feeds
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4373269
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226370
作者单位	1.厦门出入境检验检疫局, 厦门, 福建 361012, 中国; 2.中国检验检疫科学研究院, 北京 100025, 中国
推荐引用方式 GB/T 7714	严丽娟,张峰,吴敏,等. UPLC - ESI MS/MS法测定动物饲料中苯二氮卓类药物[J],2011,30(11):1301-1305.
APA	严丽娟,张峰,吴敏,吴抒怀,储晓刚,&周昱.(2011).UPLC - ESI MS/MS法测定动物饲料中苯二氮卓类药物.分析测试学报,30(11),1301-1305.
MLA	严丽娟,et al."UPLC - ESI MS/MS法测定动物饲料中苯二氮卓类药物".分析测试学报 30.11(2011):1301-1305.