干旱区生态环境知识资源中心(Arid): HPLC--MS/MS法同时测定果蔬中6种植物生长抑制剂残留

Arid

	HPLC--MS/MS法同时测定果蔬中6种植物生长抑制剂残留
其他题名	Determination of 6 Plant Growth Retardants in Fruits and Vegetables by HPLC - MS/MS
	王静静 1; 鹿毅 2; 杨涛 2; 张煌涛 2; 王吉德 1
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2011
卷号	30 期号:2 页码:128-134
中文摘要	利用高效液相色谱一电喷雾串联质谱(HPLc--ESI Ms/Ms)技术, 建立了果蔬中氯化胆碱、矮壮素、缩节胺、嘧啶醇、多效唑、烯效唑6种植物生长抑制剂残留的检测方法. 考察了流动相组分和流动相添加剂对质谱离子化效率的影响以及提取溶剂、提取剂用量和固相萃取柱对萃取效率的影响. 在优化条件下, 6种目标化合物在1.0~200.0 mug/L范围内线性良好(r>0.99), 空白样品中, 加标水平为2.0、10.0、50.0mug/kg时, 6种目标化合物的平均加标回收率为81％~104％, 相对标准偏差均不大于5.0％, 方法的检出限为 0.15~0.35mug/kg, 定量下限为0.47~1.10mug/kg. 该方法具有灵敏度高、选择性好、回收率高且稳定等优点, 用于实际果蔬样品中植物生长抑制剂的测定, 结果满意
英文摘要	A comprehensive analytical method based on high performance liquid chromatography -electrospray ionization tandem mass spectrometry (HPLC - ESI MS/MS) under positive ionization mode was developed for the determination of 6 plant growth retardants (PGRs) residues, including choline chloride, chlormequat chloride, mepiquat chloride, ancymidol, paclobutrazol and uniconazole in fruits and vegetables. The samples were extracted with methanol by ultrasonic wave assistant extraction(UAE). The extract was purified and concentrated by solid-phase extraction(SPE) with Waters Oasis HLB column(3 mL, 60 mg). The separation of the target compound was performed with Thermo Hypersil Gold aQ(2. 1 mm *150 mm, 3 mum) column by gradient elution. The identification of 6 PGRs was carried out by ESI under the positive-ion mode. Effects of mobile phase and additives on ionization were investigated. Effects of the types and amounts of extraction solvents and the types of SPE column were also studied. Under the optimal conditions, the calibration curves of 6 PGRs were linear in the range of 1. 0 -200. 0 mug/L with correlation coefficients(r) more than 0. 99. Recoveries of 6 PGRs at three spiked concentration levels of 2. 0, 10. 0, 50. 0 mug/kg ranged from 81% to 104% with relative standard deviations no more than 5. 0%. The detection limits (LOD) were in the range of 0. 15 -0. 35 mug/kg and the quantitative limits(LOQ) were 0. 47 - 1. 10 mug/kg. The established method has the advantages of high sensitivity, nice selectivity and low detection limit, and was applied in the determination of PGRs in fruits and vegetables with satisfactory results
中文关键词	高效液相色谱一电喷雾质谱/质谱 ; 植物生长抑制剂 ; 残留
英文关键词	high liquid chromatography - electrospray ionization tandem mass spectrometry (HPLC -ESI - MS/MS) plant growth retardants(PGRs) residues
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL ; BUSINESS FINANCE
WOS研究方向	Chemistry ; Business & Economics
CSCD记录号	CSCD:4150419
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226367
作者单位	1.石油天然气精细化工教育部自治区重点实验室, 石油天然气精细化工教育部自治区重点实验室, 乌鲁木齐, 新疆 830046, 中国; 2.新疆产品质量监督检验研究院, 乌鲁木齐, 新疆 830004, 中国
推荐引用方式 GB/T 7714	王静静,鹿毅,杨涛,等. HPLC--MS/MS法同时测定果蔬中6种植物生长抑制剂残留[J],2011,30(2):128-134.
APA	王静静,鹿毅,杨涛,张煌涛,&王吉德.(2011).HPLC--MS/MS法同时测定果蔬中6种植物生长抑制剂残留.分析测试学报,30(2),128-134.
MLA	王静静,et al."HPLC--MS/MS法同时测定果蔬中6种植物生长抑制剂残留".分析测试学报 30.2(2011):128-134.