干旱区生态环境知识资源中心(Arid): 超高效液相色谱-串联质谱法同时测定尿液中16种抗生素

Arid

	超高效液相色谱-串联质谱法同时测定尿液中16种抗生素
其他题名	Simultaneous Determination of Sixteen Antibiotics in Human Urine with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
	陈聪 1; 严慧 2; 沈保华 2; 卓先义 2
来源期刊	法医学杂志
ISSN	1004-5619
出版年	2011
卷号	27 期号:1 页码:25-29
中文摘要	目的建立同时分析尿液中16种抗生素的超高效液相色谱-串联质谱(UPLC-MS/MS)方法。方法以哌拉西林为内标,尿样中的目标化合物经Oasis HLB固相萃取柱富集、纯化,利用ZORBAX SB-C_(18)色谱柱,以0.1%的甲酸溶液-乙腈为流动相经梯度洗脱分离,采用多反应监测(MRM)模式进行分析。结果各化合物的最低检出限(LOD)为0.05~10.0 ng/mL,定量限(LOQ)为0.25~20.0 ng/mL。在相应线性范围内,16种抗生素的相关系数(r)均大于0.995。方法准确度为82.0%~119.3%,日内精密度均小于13.9%。结论所建方法灵敏度高、选择性好,可用于法医毒物分析和临床药物监测中的抗生素测定。
英文摘要	Objective To develop a method for simultaneous determination of sixteen antibiotics in human urine by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Methods With Piperacillin as an internal standard, the target antibiotics in urine samples were enriched and purified by Oasis HLB solid phase extraction(SPE) cartridges, then separated in a ZORBAX SB-C_(18) column with a gradient elution of mobile phase of 0.1% formic acid water and acetonitrile, finally analyzed with multiple reaction monitoring(MRM) mode. Results The limits of detection(LOD) for these sixteen antibiotics were in the range of 0.05-10.0 ng/mL and the limits of quantification(LOQ) in the range of 0.25-20.0 ng/mL. Within the related linear range, the related coefficient (r) of sixteen antibiotics were all more than 0.995. Accuracies for these antibiotics were ranged from 82.0%-119.3%, the within-day precision were less than 13.9%. Conclusion The developed method is sensitive, specific and appropriate for the analysis of antibiotics in forensic toxicology and therapeutic drugs monitoring.
中文关键词	法医毒理学 ; 抗生素 ; 尿 ; 超高效液相色谱-串联质谱法
英文关键词	forensic toxicology antibiotics urine ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)
语种	中文
国家	中国
收录类别	CSCD
WOS类目	POLITICAL SCIENCE
WOS研究方向	Government & Law
CSCD记录号	CSCD:4379736
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/226365
作者单位	1.复旦大学上海医学院法医学系, 上海市法医学重点实验室, 上海 200032, 中国; 2.司法部司法鉴定科学技术研究所, 上海市法医学重点实验室, 上海 200063, 中国
推荐引用方式 GB/T 7714	陈聪,严慧,沈保华,等. 超高效液相色谱-串联质谱法同时测定尿液中16种抗生素[J],2011,27(1):25-29.
APA	陈聪,严慧,沈保华,&卓先义.(2011).超高效液相色谱-串联质谱法同时测定尿液中16种抗生素.法医学杂志,27(1),25-29.
MLA	陈聪,et al."超高效液相色谱-串联质谱法同时测定尿液中16种抗生素".法医学杂志 27.1(2011):25-29.