干旱区生态环境知识资源中心(Arid): 饲料中螺旋霉素反相HPLC检测方法的建立

Arid

	饲料中螺旋霉素反相HPLC检测方法的建立
其他题名	The establishment of RP-HPLC method for determination of spiramycin in feeds
	张文丽; 鲍恩东
来源期刊	扬州大学学报. 农业与生命科学版
ISSN	1671-4652
出版年	2010
卷号	31 期号:3 页码:20-24
中文摘要	利用固相萃取-高效液相色谱法建立饲料中螺旋霉素的检测方法,以便确定其检测标准.饲料经甲醇提取, Oasis HLB固相萃取柱富集净化后,确定以乙腈与磷酸二氢钠溶液(体积比为22:78)作为流动相,反相高效液相色谱-紫外检测法检测,检测波长为232 nm.其检测方法中螺旋霉素在0.120.0mug.mL-1范围内有良好的线性关系;饲料中螺旋霉素的平均回收率为86%93%,变异系数小于4%;定量限为0.8 mg.kg-1,检测限为0.2 mg.kg-1;方法专属性好.结果证明该方法灵敏度高,准确性和重现性良好
英文摘要	To develop a standard determination of Spiramycin in feeds,a solid-phase extraction-high performance liquid chromatography(SPE-HPLC) method was established in this study. After extracted by methanol, concentrated and cleaned by Oasis HLB SPE column, Spiramycin was separated through RP-HPLC and detected by the UV detector under 232 nm, using acetonitrile and sodium dihydrogen phosphate buffer(22:78) as HPLC mobile phase. By RP-HPLC method the linear relationships of spiramycin were fine between 0.1-20.0 mug·mL(-1);The average recoveries of spiramycin ranged from 86%-93%, with the RSD lower than 4%;The limit of quantitation was 0.8 mg·kg(-1), and the limit of detection was 0.2 mg·kg(-1);The results indicated that the specificity of the established RP-HPLC method was good. The method had a high sensitivity, good accuracy and precision
中文关键词	螺旋霉素 ; 饲料 ; 固相萃取 ; 反相高效液相色谱
英文关键词	spiramycin feeds solid-phase extraction reverse rhase high performance liquid chromatography
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:4052249
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/225833
作者单位	南京农业大学动物医学院, 南京, 江苏 210095, 中国
推荐引用方式 GB/T 7714	张文丽,鲍恩东. 饲料中螺旋霉素反相HPLC检测方法的建立[J],2010,31(3):20-24.
APA	张文丽,&鲍恩东.(2010).饲料中螺旋霉素反相HPLC检测方法的建立.扬州大学学报. 农业与生命科学版,31(3),20-24.
MLA	张文丽,et al."饲料中螺旋霉素反相HPLC检测方法的建立".扬州大学学报. 农业与生命科学版 31.3(2010):20-24.