干旱区生态环境知识资源中心(Arid): 水产品中四环素类药物残留的超高效液相色谱-串联质谱法测定

Arid

	水产品中四环素类药物残留的超高效液相色谱-串联质谱法测定
其他题名	Determination of Tetracycline Residues in Aquatic Products by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
	林荆; 张金虎; 郑宇; 杨勇; 余钗; 吴文凡
来源期刊	食品科学
ISSN	1002-6630
出版年	2010
卷号	31 期号:20 页码:286-289
中文摘要	建立同时测定水产品中6种四环素类药物残留的超高效液相色谱-串联质谱(UPLC-MS/MS)分析方法.用0.01mol/L Na2EDTA-Mcllvaine磷酸盐缓冲溶液提取,样液过滤后正己烷除脂,经Oasis HLB固相萃取柱净化,甲醇洗脱,氮气吹干缓冲液定容后,用反相液相色谱分离,电喷雾正离子模式离子化,用多反应监测模式(MRM)监测,三重四级杆质谱测定,外标法定量.该法对四环素类药物在5.0~50.0mug/kg范围内呈线性关系,相关系数r在0.9969~0.9999之间,在5.0,10.0,25.0,50.0mug/kg添加水平,回收率为61%~97%,相对标准偏差(RSD)为1.3%~5.7%,其检出限达2.5mug/kg,方法操作简便,稳定性好,选择性好,灵敏度高
英文摘要	An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the simultaneous determination of six tetracylines in aquatic products. Samples were extracted with 0.01 mol/L Na2EDTA/Mcllvaine buffer solution. After filtrated and degreased by n-hexane,the extract was passed through Oasis HLB solid phase extraction column and eluted with methanol,and the eluate was then pooled together and evaporated until dryness under nitrogen and redissolved in 1.0 mL of dissolving solution. The solution was separated on a RP-UPLC column and detected by triple-quadrupole MS. The electrospraying was operated in the positive ionization mode and the identification of analytes was achieved using multiple reaction monitoring (MRM) mode. Quantitation was carried out using the external standard method. The standard curves for all the compounds were linear over the concentration range of 5.0 to 50.0mug/kg with correlation coefficients between 0.9969 and 0.9999. The spike recoveries at four levels of 5.0,10.0,25.0,50.0mug/kg ranged form 61% to 97%,and the relatives standard deviations (RSD) were in the range of 1.3% to 5.7%. The limits of detection of the method for the analytes were all 2.5 mu g/kg. Owing to the merits of simplicity,selectivity and sensitivity,the proposed method has been successfully applied to the determination of tetracyclines in aquatic products
中文关键词	水产品 ; 四环素类抗生素 ; 超高效液相色谱-串联质谱
英文关键词	aquatic products tetracyclin antibiotics ultra performance liquid chromatography-tandem mass spectrom-etry (UPLC-MS/MS)
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4105126
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/225592
作者单位	福清出入境检验检疫局, 福清, 福建 350300, 中国
推荐引用方式 GB/T 7714	林荆,张金虎,郑宇,等. 水产品中四环素类药物残留的超高效液相色谱-串联质谱法测定[J],2010,31(20):286-289.
APA	林荆,张金虎,郑宇,杨勇,余钗,&吴文凡.(2010).水产品中四环素类药物残留的超高效液相色谱-串联质谱法测定.食品科学,31(20),286-289.
MLA	林荆,et al."水产品中四环素类药物残留的超高效液相色谱-串联质谱法测定".食品科学 31.20(2010):286-289.