干旱区生态环境知识资源中心(Arid): 内蒙古退化荒漠草原土壤细菌群落结构特征

Arid

	内蒙古退化荒漠草原土壤细菌群落结构特征
其他题名	Characteristics of bacterial community structure in degraded desert steppe of Inner Mongolia
	吴永胜 1; 马万里 2; 李浩 2; 卢萍 2; 吕桂芬 2
来源期刊	生态学报
ISSN	1000-0933
出版年	2010
卷号	30 期号:23 页码:6355-6362
中文摘要	用PCR-DGGE技术对不同退化程度荒漠草地土壤细菌群落结构的分析表明:不同退化草地土壤细菌群落结构发生了明显的变化.2006年夏季,不同退化草地土壤细菌的Shannon-Weaver指数随退化程度的加剧呈降低趋势,由高到低依次是:轻度退化,中度退化和重度退化草地.2007年夏季多样性指数随退化程度的加剧呈先降低后增加的趋势,由高到低依次为:轻度退化,重度退化和中度退化.对17个克隆进行了序列测定所得到的16SrDNA在数据库中的登录号为:EU327142-EU327157和EU327164.将其与Genbank数据库中的序列进行比对,结果表明这些序列与已知序列的相似性均在95%-99%之间.系统发育分析结果表明,内蒙古荒漠草地土壤主要细菌隶属于以下几个分支:拟杆菌纲(Bacteroidetes),酸杆菌纲(Acidobacteria),变形菌纲(Proteobacteria)的gamma,delta类群和厚壁菌纲(Firmicutes).其中,拟杆菌纲所占的比例为47%左右,为优势种
英文摘要	Soil microorganisms play key roles in soils through regulating organic matter decomposition and plant nutrient availability.However,due to the complexity of microorganism survival condition,only 1%-5% of the total microorganisms can be isolated by cultural method in soils.Recently,the advances of molecular biological techniques can provide information regarding soil bacterial community structure through culture-independent approaches.To better understand the impact of grassland degradation on the composition and structure of soil bacterial community,the total DNA of microorganisms was extracted directly and amplified by the 16S rRNA gene specific primers of f954 with GC clamp and 1369r,and the changes of the soil bacterial community structure in degraded area(including light,moderate and heavy degraded) of desert steppe ecosystem in Inner Mongolia were determined by the PCR-DGGE method.Results indicate that soil bacterial community structures in degraded desert steppe changed significantly.In 2006 summer,Shannon-Weaver Indices decreased as increasing of degradation degree,ie.,the highest in the light degradation area,followed with moderate degraded area,and the least in heavy degradation area.In 2007 summer,they decreased initially and then increased,ie.,in the light,heavy and moderate degradation areas,they decreased orderly.Seventeen 16S rDNA clones were sequenced and their accession numbers in the data bank were EU327142-EU327157 and EU327164.Compared with deposited in the Genbank(www.ncbi.nlm.nih.gov),it shows that their level of nucleotide identity changed with a range from 95 to 100%.The phylogenetic affiliation of bacteria determined by 16S rDNA sequencing of cloned DNA fragments in different degraded desert steppe indicate that they were mainly attributed to Bacteroidetes,Acidobacteria,gamma,delta groups of Proteobacteria and Firmicutes and in which Bacteroidetes were dominant with 47%
中文关键词	荒漠草原 ; 土壤细菌 ; 群落结构
英文关键词	PCR-DGGE desert steppe soil bacteria community structure PCR-DGGE
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENVIRONMENTAL SCIENCES
WOS研究方向	Environmental Sciences & Ecology
CSCD记录号	CSCD:4081114
来源机构	北京师范大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/225544
作者单位	1.北京师范大学, 地表过程与资源生态国家重点实验室, 北京 100875, 中国; 2.内蒙古师范大学生命科学与技术学院, 呼和浩特, 内蒙古 010022, 中国
推荐引用方式 GB/T 7714	吴永胜,马万里,李浩,等. 内蒙古退化荒漠草原土壤细菌群落结构特征[J]. 北京师范大学,2010,30(23):6355-6362.
APA	吴永胜,马万里,李浩,卢萍,&吕桂芬.(2010).内蒙古退化荒漠草原土壤细菌群落结构特征.生态学报,30(23),6355-6362.
MLA	吴永胜,et al."内蒙古退化荒漠草原土壤细菌群落结构特征".生态学报 30.23(2010):6355-6362.