干旱区生态环境知识资源中心(Arid): 超高效液相色谱-电喷雾串联质谱法测定动物饲料中的大环内酯类和林可胺类抗生素

Arid

	超高效液相色谱-电喷雾串联质谱法测定动物饲料中的大环内酯类和林可胺类抗生素
其他题名	Simultaneous determination of macrolide and lincosamide antibiotics in animal feeds by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry
	严丽娟 1; 张峰 2; 方恩华 1; 郭彦妮 1; 周昱 1; 林立毅 1; 储晓刚 2
来源期刊	色谱
ISSN	1000-8713
出版年	2010
卷号	28 期号:11 页码:1038-1042
中文摘要	建立了动物饲料中竹桃霉素,红霉素,吉他霉素,交沙霉素,罗红霉素,泰乐菌素6种大环内酯和林可霉素,克林霉素2种林可胺抗生素的超高效液相色谱-电喷雾串联质谱(UPLC-ESI-MS/MS)检测方法.饲料样品采用甲醇提取, Oasis HLB固相萃取柱富集净化, Waters Acquity UPLCBEHC18色谱柱分离,以0.1%甲酸和乙腈为流动相进行梯度洗脱,流速为0.3mL/min,正离子模式扫描,多反应监测模式检测,外标法定量.实验结果表明,8种药物在1~100mug/L范围内具有良好的线性关系.在空白饲料样品中分别添加1,10和100mug/kg3个加标水平的8种药物,其平均回收率为68.6%~95.2%,相对标准偏差(RSD)为4.9%~11.8%,定量限均为1mug/kg.结果表明,该方法简便快速,灵敏度高,适用于动物饲料中大环内酯类和林可胺类抗生素的同时检测
英文摘要	A method for the simultaneous determination of six macrolide antibiotics(oleandomycin, erythromycin, kitasamycin, josamycin, roxithromycin and tylosin)and two lincosamide antibiotics(lincomycin and clindamycin)in animal feeds by ultra-performance liquid chromatographyelectrospary ionization tandem mass spectrometry(UPLC-ESI-MS/MS)was developed. The macrolide and lincosamide antibiotics were extracted from the feeds with methanol followed by enrichment and clean-up with an Oasis HLB cartridge. The UPLC separation was performed on a Waters Acquity UPLC BEH C18column by agradient elution using 0.1%formic acid and acetonitrile as the mobile phase at a flow rate of 0.3 mL/min. The identification of eight drugs was carried out by positive electrospray ionization in multiple reaction monitoring(MRM)mode, and the quantification analysis was performed by external standard method. The calibration curves showed good linearity in the range of 1-100mug/L.The average recoveries of the eight drugs from the feeds spiked at 1,10 and 100mug/kg levels were between 68.6%and 95.2%, and the relative standard deviations(RSD)were between 4.9%and 11.8%.The limits of quantification(LOQ)of the drugs in the feeds were 1mug/kg. The method is simple, rapid, sensitive and suitable for the simultaneous determination of macrolide and lincosamide antibiotics in animal feeds
中文关键词	超高效液相色谱-串联质谱 ; 大环内酯类抗生素 ; 林可胺类抗生素 ; 饲料
英文关键词	ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) macrolide antibiotics lincosamide antibiotics feeds
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:4070602
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/225452
作者单位	1.厦门出入境检验检疫局, 厦门, 福建 361012, 中国; 2.中国检验检疫科学研究院, 北京 100025, 中国
推荐引用方式 GB/T 7714	严丽娟,张峰,方恩华,等. 超高效液相色谱-电喷雾串联质谱法测定动物饲料中的大环内酯类和林可胺类抗生素[J],2010,28(11):1038-1042.
APA	严丽娟.,张峰.,方恩华.,郭彦妮.,周昱.,...&储晓刚.(2010).超高效液相色谱-电喷雾串联质谱法测定动物饲料中的大环内酯类和林可胺类抗生素.色谱,28(11),1038-1042.
MLA	严丽娟,et al."超高效液相色谱-电喷雾串联质谱法测定动物饲料中的大环内酯类和林可胺类抗生素".色谱 28.11(2010):1038-1042.