干旱区生态环境知识资源中心(Arid): 超高效液相色谱法测定青娥丸中松脂醇二葡萄糠苷的含量

Arid

	超高效液相色谱法测定青娥丸中松脂醇二葡萄糠苷的含量
其他题名	Determination of pinoresinol diglucoside in Qing’e Pills by ultra performance liquid chromatography
	张紫佳 1; 陈洁 1; 赵骏铭 1; 廖立平 1; 孙庆龙 2; 吴弢 1; 王峥涛 1
来源期刊	色谱
ISSN	1000-8713
出版年	2010
卷号	28 期号:8 页码:805-808
中文摘要	建立了超高效液相色谱(UPLC)测定青娥丸中主要活性成分松脂醇二葡萄糖苷(pinoresinol diglucoside, PDG)含量的方法. 样品经索氏提取后, 提取物再用Waters Oasis HLB SPE固相萃取小柱进行前处理以消除杂质对PDG色谱峰的干扰. 色谱条件: 采用Waters Acquity C18.BEH UPLC柱(100 mm*1.0 mm, 1.7mum)分离, 以乙腈-水(使用磷酸调pH值至4.O)(9: 91, v/v)为流动相, 流速为0.1 mL/rain, 检测波长为227 nm, 柱温为25℃, 进样量为O.5muL. 在上述条件下, 松脂醇二葡萄糖苷在1.40~506.00 mg/L范围内呈现良好的线性关系, 相关系数r=1; 低、中、高3个添加水平下的平均回收率分别为100.51％、102.37％、100.10％(n=9). 该方法准确、灵敏、重现性好, 可用于青娥丸的质量控制
英文摘要	A new ultra performance liquid chromatographic (UPLC) method was established for the determination of pinoresinol diglucoside (PDG) in Qing’ e Pills. After extracted by the Soxhlet’ s method, the methanol extracts of the samples were passed through a Waters Oasis HLB SPE column to achieve good chromatographic performance. The separation was performed on a Waters Acquity C18 BEH column (100 mm * 1.0 mm, 1.7 mum) with acetonitrile-water (the pH adjusted to 4. 0 with phosphoric acid) (9: 91, v/v) as the mobile phase at a flow rate of 0. 1 mL/min. The detection wavelength was set at 227 nm, the column temperature was 25 ℃ and the injection volume was 0. 5 muL. Under the optimized conditions, there was good linear relationship between the mass concentration and the peak area of PDG in the range of 1.40 -506. 00 mg/L with the correlation coefficient of 1. The average recoveries of PDG at three levels ranged from 100. 10% to 102. 37%. The method is accurate, sensitive, highly reproducible and suitable for the quality control of Qing’ e Pills
中文关键词	超高效液相色谱 ; 松脂醇二葡萄糖苷 ; 青娥丸
英文关键词	ultra performance liquid chromatography (UPLC) pinoresinol diglucoside Qing’ e Pill
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3976476
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/225450
作者单位	1.上海中医药大学中药研究所, 中药标准化教育部重点实验室;;中药新资源与质量标准综合评价国家中医药管理局重点研究室, 上海 201210, 中国; 2.沃特世科技(上海)有限公司, 上海 200233, 中国
推荐引用方式 GB/T 7714	张紫佳,陈洁,赵骏铭,等. 超高效液相色谱法测定青娥丸中松脂醇二葡萄糠苷的含量[J],2010,28(8):805-808.
APA	张紫佳.,陈洁.,赵骏铭.,廖立平.,孙庆龙.,...&王峥涛.(2010).超高效液相色谱法测定青娥丸中松脂醇二葡萄糠苷的含量.色谱,28(8),805-808.
MLA	张紫佳,et al."超高效液相色谱法测定青娥丸中松脂醇二葡萄糠苷的含量".色谱 28.8(2010):805-808.