干旱区生态环境知识资源中心(Arid): 液相色谱-串联质谱法快速测定蜂蜜中3种硝基咪唑类药物残留

Arid

	液相色谱-串联质谱法快速测定蜂蜜中3种硝基咪唑类药物残留
其他题名	Rapid determination of 3nitromidazole residues in honey using liquid chromatography-tandem mass spectrometry
	刘永明; 曹彦忠; 李金; 吴艳萍; 张进杰; 李学民; 葛娜
来源期刊	色谱
ISSN	1000-8713
出版年	2010
卷号	28 期号:6 页码:596-600
中文摘要	建立了液相色谱-串联质谱(LC-MS/MS)快速测定蜂蜜中甲硝唑、洛硝哒唑和二甲硝咪唑3种硝基咪唑类药物残留的分析方法. 蜂蜜样品用水溶解后,直接上样至Oasis HLB固相萃取柱净化,依次用水和甲醇-水溶液(1:9, v/v)淋洗,用乙酸乙酯洗脱. 洗脱液经浓缩、溶解、过滤后进行LC-MS/MS检测,外标法定量. 在添加水平为0.05 ~2.0mug/kg时,平均添加回收率为76.6%~89.7%,相对标准偏差(n=8)为5.2%~9.9%. 甲硝唑的检出限为 0.1mug/kg,洛硝哒唑、二甲硝咪唑的检出限均为0.2mug/kg. 应用所建立的方法对出口蜂蜜样品进行了测定,结果表明该方法操作简单、快速,结果准确、可靠,灵敏度和准确度满足现在日本和欧盟对蜂蜜中3种硝基咪唑类药物残留量的检测要求
英文摘要	A method was developed for the rapid determination of 3 nitromidazole residues in honey, including metronidazole, ronidazole and dimetridazole, using liquid chromatographytandem mass spectrometry (LC-MS/MS). The honey sample was dissolved in water, and the solution was cleaned up with an Oasis HLB cartridge. The cartridge was washed with water and methanol-water solution (1:9, v/v) in turn, and eluted with ethyl acetate. The solution was concentrated and then analyzed by LC-MS/MS. External calibration was used for quantitative determination. The recoveries and relative standard deviations (n = 8) were from 76. 6% to 89. 7% and 5. 2% to 9. 9%, at the spiked levels of 0. 05 - 2. 0 mug/kg, respectively. The limits of detection were 0. 1 mug/kg for metronidazole, and 0. 2 mug/kg for ronidazole and dimetridazole. The method was successfully applied for the inspection of exported honey. With simple and fast sample preparation, the method is sensitive and specific for the determination. The sensitivity and accuracy of the method meet the requirements of the inspection for the 3 nitromidazole residues in honey in Japan and European Union
中文关键词	液相色谱-串联质谱法 ; 甲硝唑 ; 洛硝哒唑 ; 二甲硝咪唑 ; 蜂蜜
英文关键词	liquid chromatography-tandem mass spectrometry (LC-MS/MS) metronidazole ronidazole dimetridazole honey
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3908961
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/225448
作者单位	秦皇岛出入境检验检疫局, 秦皇岛, 河北 066004, 中国
推荐引用方式 GB/T 7714	刘永明,曹彦忠,李金,等. 液相色谱-串联质谱法快速测定蜂蜜中3种硝基咪唑类药物残留[J],2010,28(6):596-600.
APA	刘永明.,曹彦忠.,李金.,吴艳萍.,张进杰.,...&葛娜.(2010).液相色谱-串联质谱法快速测定蜂蜜中3种硝基咪唑类药物残留.色谱,28(6),596-600.
MLA	刘永明,et al."液相色谱-串联质谱法快速测定蜂蜜中3种硝基咪唑类药物残留".色谱 28.6(2010):596-600.