干旱区生态环境知识资源中心(Arid): 高效液相色谱-串联质谱测定猪肉中3种beta-受体激动剂残留量

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	高效液相色谱-串联质谱测定猪肉中3种beta-受体激动剂残留量
其他题名	Determination of 3 beta-agonist residues in pork by high performance liquid chromatography-electrospray ionization tandem mass spectrometry
	郭伟; 杨丽君; 时文春; 徐成钢; 崔佳; 李兆杰; 夏凡
来源期刊	分析试验室
ISSN	1000-0720
出版年	2010
卷号	29 期号:4 页码:92-98
中文摘要	建立一种同时测定猪肉中3种beta-受体激动剂残留量的高效液相色谱-电喷雾串联质谱(HPLC-ESI-MS/MS)确证分析方法.样品经beta-葡萄糖醛酸酶/芳基硫酸酯酶酶解、乙酸铵缓冲液提取和MCX固相萃取柱净化,采用Agilent Zorbax SB-C_(18) (2.1 mm*150 mm,3.5 mum)色谱柱,0.1%的甲酸水溶液、甲醇和乙腈作为流动相进行洗脱,高效液相色谱分离,电喷雾离子源电离,正离子多反应监测模式进行检测,内标法定量.3种药物在0.05～1 mug/kg浓度范围内线性良好,相关系数r均大于0.999,0.05、0.1、0.5 mug/kg 3个浓度水平的添加回收率在89.7%～106.7%之间,相对标准偏差为2.4%～8.6%,3种药物的定量限均为0.05 mug/kg.方法适用于猪肉中beta-受体激动剂残留的确证分析.
英文摘要	A confirmative method was developed by high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) to detect 3 beta-agonist residues in pork simultaneously, which included clenbuterol, ractopamine, salbutamol. In the method, The analytes were extracted with NH4Ac solution from homogenized samples and hydrolysised with beta-glucuronidase overnight at 37℃. Then the extract was filtered and the filtrate was cleaned by oasis MCX solid phase extraction cartridge. The HPLC separation was performed on an agilent zorbax SB-C_(18) column (2.1 mm*150 mm, 3.5 mum) utilizing a isocratic elution program of water (containing 0.1% formic acid), methanol and acetonitrile as the mobile phase. Identification and quantification were achieved by using HPLC-ESI-MS/MS in positive mode and multiple reaction monitoring (MRM) using internal standard method. Good linearities were observed in the range from 0.05 mug/kg to 1 mug/kg with correlation coefficients above 0.999. The method was validated at 0.05, 0.10 and 0.50 mug/kg. The validation results were as follows: the average recoveries of 3 beta-agonists ranged from 89 7% to 106.7% with the relative standard deviations of 2.4%～8.6%, quantification limits for the three analytes were all 0 05 mug/kg. With good reproducibility, high sensitivity, short analysis time and good determination capacity, the method could meet the requirements of both domestic and international legislation. The method is applicable to simultaneous confirmation of multi-residues of 3 beta-agonists in pork.
中文关键词	高效液相色谱-电喷雾串联质谱 ; beta-受体激动剂 ; 猪肉
英文关键词	High performance liquid chromatography-electrospray ionization tandem mass spectrometry Beta-agonist Pork
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3844905
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/224925
作者单位	威海出入境检验检疫局技术中心, 威海, 山东 264205, 中国
推荐引用方式 GB/T 7714	郭伟,杨丽君,时文春,等. 高效液相色谱-串联质谱测定猪肉中3种beta-受体激动剂残留量[J],2010,29(4):92-98.
APA	郭伟.,杨丽君.,时文春.,徐成钢.,崔佳.,...&夏凡.(2010).高效液相色谱-串联质谱测定猪肉中3种beta-受体激动剂残留量.分析试验室,29(4),92-98.
MLA	郭伟,et al."高效液相色谱-串联质谱测定猪肉中3种beta-受体激动剂残留量".分析试验室 29.4(2010):92-98.