干旱区生态环境知识资源中心(Arid): 液相色谱-串联质谱法测定牛奶和奶粉中卡巴氧和喹乙醇代谢物的残留量

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	液相色谱-串联质谱法测定牛奶和奶粉中卡巴氧和喹乙醇代谢物的残留量
其他题名	Determination of the residues of metabolites of carbadox and olaquindox in milk and milk powder-LC-MS-MS method
	林黎 1; 谢丽琪 1; 欧阳姗 1; 梁宏 1; 叶刚 1; 廖菁菁 1; 庞国芳 2
来源期刊	分析试验室
ISSN	1000-0720
出版年	2010
卷号	29 期号:2 页码:38-41
中文摘要	建立了牛奶和奶粉中卡巴氧代谢物喹喔啉-2-羧酸(QCA)和喹乙醇代谢物3-甲基喹喔啉-2-羧酸(MQCA)残留量的液相色谱-串联质谱测定方法. 将样品经0.6% (体积分数)的甲酸溶液进行消化, 用Tris缓冲溶液调节pH后, 加入Protease蛋白酶进行酶解, 样品溶液用0.3 mol/L HCl溶液酸化后, 采用阴离子交换固相萃取柱Oasis MAX进行净化和富集. 分析样品以0.1% (体积分数)的甲酸溶液-甲醇-乙腈为流动相, 经Inertsil ODS-3色谱柱分离, 采用负离子扫描, 在LC-MS/MS多反应监测模式下进行定性及定量分析. 喹口恶啉-2-羧酸和3-甲基喹口恶啉-2-羧酸的方法测定下限牛奶为0.5 mug/kg, 奶粉为4.0 mug/kg. 对牛奶在0.5～5.0 mug/kg, 奶粉在4.0～40.0 mug/kg添加水平的平均回收率为68.2%～82.5%, RSD为3.4%～12%.
英文摘要	This study presented a liquid chromatography-tandam mass spectrometry (LC-MS/MS) method for determination of residues of Carbadox, Olaquindox and related metabolites quinoxalie-2-carboxylic acid(QCA) and 3-methylquinoxaline-2-carboxylic acid (MQCA) in milk and milk powder. As for the extraction of QCA and MQCA, milk and milk powder is first digested with 0.6% formic acid to deactivate naturally-occurring enzymes. After overnight enzymatic hydrolysis with protease, the tissue extract is acidified, centrifuged and filtered. QCA and MQCA are recovered from the tissue extract by using an Oasis MAX solid phase extraction (SPE) cartridge. The analytes are separated on a Inertsil ODS-3 LC column, and qualitatively and quantitatively determined under multi-reaction monitoring (MRM) scan type with tandem mass analyzer using positive polarity mode. The limit of quatitation (LOQ) for the two analytes in milk is 0.5 mug/kg, in milk power is 4.0 mug/kg. At the spiked level in the range of 0.5～5.0 mug/kg in milk and 4.0～40.0 mug/kg in milk power, the mean recoveries are in the range of 68.2%～82.5%, the relative standard deviation are in the range of 3.4%～12%.
中文关键词	液相色谱-串联质谱 ; 卡巴氧 ; 喹乙醇 ; 代谢产物 ; 牛奶 ; 奶粉
英文关键词	LC-MS/MS Carbadox Olaquindox Metabolites Milk Milk powder
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3807474
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/224923
作者单位	1.深圳出入境检验检疫局, 深圳, 广东 518067, 中国; 2.秦皇岛出入境检验检疫局, 秦皇岛, 河北 066000, 中国
推荐引用方式 GB/T 7714	林黎,谢丽琪,欧阳姗,等. 液相色谱-串联质谱法测定牛奶和奶粉中卡巴氧和喹乙醇代谢物的残留量[J],2010,29(2):38-41.
APA	林黎.,谢丽琪.,欧阳姗.,梁宏.,叶刚.,...&庞国芳.(2010).液相色谱-串联质谱法测定牛奶和奶粉中卡巴氧和喹乙醇代谢物的残留量.分析试验室,29(2),38-41.
MLA	林黎,et al."液相色谱-串联质谱法测定牛奶和奶粉中卡巴氧和喹乙醇代谢物的残留量".分析试验室 29.2(2010):38-41.