干旱区生态环境知识资源中心(Arid): 快速高分离液相色谱-串联质谱法检测原乳中活性beta-内酰胺酶

Arid

	快速高分离液相色谱-串联质谱法检测原乳中活性beta-内酰胺酶
其他题名	Qualitative Analysis of Active Beta-Lactamases in Milk Samples by Rapid Resolution Liquid Chromatography-Tandem Mass Spectrometry
	孙汉文 1; 李挥 1; 张敬轩 2; 周正 2
来源期刊	分析化学
ISSN	0253-3820
出版年	2010
卷号	38 期号:8 页码:1203-1205
中文摘要	建立了原乳中活性 beta -内酰胺酶拮抗剂的快速高分离液相色谱-串联质谱(RRLC-MS/MS)检测方法. 利用活性 beta -内酰胺酶能够酶解青霉素族药物的特性, 在原乳中加入适量氨苄青霉素, 酶解1 h后, 检测原乳中氨苄青霉素酶解率, 定性检测活性目标物. 试样中氨苄青霉素经乙腈溶液提取, HLB固相萃取柱净化和浓缩后, RRLC-MS/MS多反应监测模式定性与定量分析. 本方法对活性 beta -内酰胺酶检出限为4 U/mL; 对原乳中添加1 mg/kg水平氨苄青霉素的加标回收率为92.3%~95.5%; 相对标准偏差RSD≤10%( n =6). 本方法的定性结果与生物杯碟法完全一致
英文摘要	A rapid resolution liquid chromatography-tandem mass spectrometric method (RRLC-MS/MS) has been developed for the qualitative analysis of active beta-lactamases in milk samples. Penicillin was added to the samples solution to enzymolyze the active beta-lactamases. Then, the analytes were extracted by CH_3CN-H_2O from homogenized samples, and the extract was cleaned by Oasis HLB solid phase extraction cartridge. Quantification of penicillin was achieved by RRLC-MS/MS with multiple reaction monitoring. Detection limit of active beta-lactamases was 4 U/mL, the mean recoveries were in the range of 92.3%-95.5%, and the RSD was less than 10%( n =6). The results of this method were consistent with these by the cylinder plate method
中文关键词	液相色谱-串联质谱 ; 原乳 ; beta -内酰胺酶
英文关键词	Rapid resolution liquid chromatography-tandem mass spectrometry Milk beta -Lactamases
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENGINEERING CHEMICAL
WOS研究方向	Engineering
CSCD记录号	CSCD:3965367
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/224921
作者单位	1.河北大学化学与环境科学学院, 河北省分析科学重点实验室, 保定, 河北 071002, 中国; 2.河北省食品质量监督检验研究院, 石家庄, 河北 050051, 中国
推荐引用方式 GB/T 7714	孙汉文,李挥,张敬轩,等. 快速高分离液相色谱-串联质谱法检测原乳中活性beta-内酰胺酶[J],2010,38(8):1203-1205.
APA	孙汉文,李挥,张敬轩,&周正.(2010).快速高分离液相色谱-串联质谱法检测原乳中活性beta-内酰胺酶.分析化学,38(8),1203-1205.
MLA	孙汉文,et al."快速高分离液相色谱-串联质谱法检测原乳中活性beta-内酰胺酶".分析化学 38.8(2010):1203-1205.