干旱区生态环境知识资源中心(Arid): 高效液相色谱-电喷雾串联质谱法测定蜂蜜中5种头孢菌素

Arid

	高效液相色谱-电喷雾串联质谱法测定蜂蜜中5种头孢菌素
其他题名	Determination of 5 Cephalothins in Honey by High Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry
	李学民; 曹彦忠; 张进杰; 范春林; 刘晓茂; 钱小清
来源期刊	分析化学
ISSN	0253-3820
出版年	2010
卷号	38 期号:5 页码:735-739
中文摘要	建立了用高效液相色谱-电喷雾串联质谱(LC-MS-MS)测定蜂蜜中5种头孢菌素残留量的方法. 蜂蜜样品用磷酸盐缓冲溶液溶解, 经Oasis HLB固相萃取柱净化. 5种头孢菌素在C 18 柱上以乙腈-水为流动相梯度洗脱条件下完成分离, 电喷雾电离串联质谱在正离子多反应监测模式下进行测定. 添加浓度在2~100 mug/kg范围时, 回收率为84.2%~103.6%; 相对标准偏差为3.89%~9.08%. 本方法头孢唑啉的定量限为 10 mug/kg , 头孢匹林、头孢氨苄、头孢洛宁、头孢喹肟的定量限为2.0 mug/kg.
英文摘要	A sensitive method was developed for the determination of residue of 5 cephalothins in honey by high performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-MS/MS). Honey sample was dissolved in sodium dihydrogen phosphate buffer solution, then sample solution was cleaned up with Oasis HLB SPE cartridge. Identification was achieved by electrospray ionization(ESI) in positive mode. The target compounds were separated on a C18 column by gradient elution with acetonitrile and 01% formic acid solution as mobile phase and residues were quantified in multiple reaction monitoring mode. Recoveries of 5 cephalothins were between 84.2% and 102.5%(n =10) at spiked levels of 2.0- 100.0 mug/kg . The relative standard deviations(RSD) of the method were between 3.89% and 9.08%. The limit of quantitation(LOQ) of the method for cefazolin was 10.0 mug/kg, and the LOQ of the method for cephapirin, cephalexin, cefalonium and cefquinome was 2.0 mug/kg.
中文关键词	高效液相色谱-电喷雾串联质谱 ; 头孢菌素 ; 蜂蜜
英文关键词	High performance liquid chromatography coupled with electrospray tandem mass spectrometry Cephalothins Honey
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENGINEERING CHEMICAL
WOS研究方向	Engineering
CSCD记录号	CSCD:3918435
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/224920
作者单位	秦皇岛出入境检验检疫局, 秦皇岛, 河北 066004, 中国
推荐引用方式 GB/T 7714	李学民,曹彦忠,张进杰,等. 高效液相色谱-电喷雾串联质谱法测定蜂蜜中5种头孢菌素[J],2010,38(5):735-739.
APA	李学民,曹彦忠,张进杰,范春林,刘晓茂,&钱小清.(2010).高效液相色谱-电喷雾串联质谱法测定蜂蜜中5种头孢菌素.分析化学,38(5),735-739.
MLA	李学民,et al."高效液相色谱-电喷雾串联质谱法测定蜂蜜中5种头孢菌素".分析化学 38.5(2010):735-739.