干旱区生态环境知识资源中心(Arid): 大鼠尿液中异丙嗪及其主要代谢物的 GC-MS分析

Arid

	大鼠尿液中异丙嗪及其主要代谢物的 GC-MS分析
其他题名	Analysis of Promethazine and Its Metabolites in Rats Urine by Gas Chromatography - Mass Spectrometry
	黄克建 1; 朱定姬 2; 李宏森 1; 林翠梧 2; 李璐 1; 刘晓锋 1
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2010
卷号	29 期号:3 页码:220-225
中文摘要	采用气相色谱-质谱(GC-MS)分析了大鼠尿液中的异丙嗪及其代谢产物. 尿液经beta-葡萄糖醛酸酶酶解, Oasis?HLB 3 mL固相萃取柱提取后,以RestekRtx-5MS(30 m0.25 mm0.25mum)石英毛细管色谱柱为分析柱,经GC-MS分离检测. 根据质谱图对代谢物的结构进行鉴定. 结果表明:在大鼠尿液中检出了吩噻嗪、去甲基异丙嗪、异丙嗪亚砜、2-羟基去甲基异丙嗪、二羟基异丙嗪、顺-N-(1-丙烯基)吩噻嗪、反-N-(1-丙烯基)吩噻嗪、顺-N-(1-丙烯基)吩噻嗪砜、反-N-(1-丙烯基)吩噻嗪砜、顺-N-(1-丙烯基)吩噻嗪亚砜及反-N-(1-丙烯基)吩噻嗪亚砜11种代谢物. 对异丙嗪在大鼠体内的氧化、还原等主要代谢途径进行了讨论.
英文摘要	A gas chromatography and mass spectrometric( GC - MS) method was developed for the analysis of promethazine( PMZ) and its metabolites in wistar rat with the electron ionization( EI) and the positive chemical ionization(PCI) mode. The sample was hydrolyzed with beta-gl curonidase, and extracted by a Oasis? HLB 3 mL solid phase extraction( SPE). The separation of target compounds was performed on a Restek Rtx -5 MS(30 m x 0. 25 mm x 0. 25 mum) capillary column. The molecular structures of the metabolites were identified based on their MS spectra. The result indicated that PMZ and its eleven metabolites, including phenothiazine, desmethylpromethazine, promethazine sulphoxide, 2-hydroxy-desmethylpromethazine, dihydroxypromethazine, cis-N-( 1-propenyl ) phenothiazine, trans-N-( 1-propenyl) phenothiazine, cis-N-( I-propenyl) phenothiazine-5-oxide, trans-N-( 1-propenyl) phenothiazine-5-oxide, cis-N-( 1-propenyl) phenothiazine-5, 5-dioxide, trans-N-(propenyl) phenothiazine-5, 5-dioxide were found in rat urine. The main metabolic process of PMZ in rat bio-body was also discussed.
中文关键词	异丙嗪 ; 尿液 ; 代谢物 ; 气相色谱-质谱
英文关键词	promethazine urine metabolites GC - MS
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3925794
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/224910
作者单位	1.广西壮族自治区公安厅物证鉴定中心, 南宁, 广西 530012, 中国; 2.广西大学化学化工学院, 南宁, 广西 530004, 中国
推荐引用方式 GB/T 7714	黄克建,朱定姬,李宏森,等. 大鼠尿液中异丙嗪及其主要代谢物的 GC-MS分析[J],2010,29(3):220-225.
APA	黄克建,朱定姬,李宏森,林翠梧,李璐,&刘晓锋.(2010).大鼠尿液中异丙嗪及其主要代谢物的 GC-MS分析.分析测试学报,29(3),220-225.
MLA	黄克建,et al."大鼠尿液中异丙嗪及其主要代谢物的 GC-MS分析".分析测试学报 29.3(2010):220-225.