干旱区生态环境知识资源中心(Arid): 鸡肌肉组织中四环素类和氟喹诺酮类药物多残留检测

Arid

	鸡肌肉组织中四环素类和氟喹诺酮类药物多残留检测
其他题名	Determination of tetracyclines and fluoroquinolones multiresidue in chicken muscle by High-Liquid Chromatography
	郭黎明; 朱奎; 丁双阳; 江海洋; 李建成; 李晓薇
ISSN	1007-4333
出版年	2009
卷号	14 期号:5 页码:98-102
中文摘要	建立了能同时提取净化鸡肌肉组织中多残留的前处理并使用高效液相色谱分别检测的方法.样品经McIlvaine缓冲液、McIlvaine缓冲液-甲醇混合液提取后,过Oasis HLB固相萃取柱,5%甲醇(psi)淋洗,甲醇洗脱,40℃氮气吹干,磷酸-三乙胺缓冲液/乙腈复溶.样品四环素类药物用紫外检测器检测,波长365 nm,氟喹诺酮类药物用荧光检测器检测:激发波长280 nm,发射波长为480 nm.结果显示:鸡肌肉组织中4种四环素类药物OTC、TC的检测限是5mug/kg,CTC、DOXY的检测限是16 mug/kg,回收率范围60.6%～89.1%,变异系数范围1.9%～14.3%;4种氟喹诺酮类药物的检测限均为2 mug/kg,回收率范围77.5%～86.3%,变异系数范围2.8%～7.4%.说明该方法灵敏度高、适应性强,适用于鸡肌肉组织中上述4种四环素类药物和4种氟喹诺酮类药物残留的一次性检出.
英文摘要	In this study,the multiresidue of oxytetracycline(OTC),tetracycline(TC),chlortetracycline(CTC),doxytetracycline(DOXY),ofloxacin(OFL),norfloxacin(NOR),ciprofloxacin(CIP) and enrofloxacin(ENR) were simultaneously extraction and purification from chicken muscle by HPLC.The homogenized samples were extracted with perchloric acid to remove proteins and the extracts were partitioned with McIlvaine buffer and McIlvaine-methanol buffer.Further cleanup was perfomed on Oasis HLB solid-phase column.The cartridge was washed with 5% methanol,eluted with methanol.The elution was vaporized to dryness with nitrogen at 40℃,and the dry residue was resolved in phosphoric acid-triethylamine-acetonitrile.Then the multriresidues were determined with UV detector at 365 nm and fluoroquinolones were determined by fluorescences detector with excitation at 280 nm,emission at 480 nm.Limits of detection(LOD) of OTC and TC were 5 mug/kg,CTC or DOXY were 16 mug/kg,the recoveries and the coefficient of variation(CV) of tetracyclines were 60.6%-89.1% and 1.9%-14.3% respectively.LOD of fluoroquinolones were 2 mug/kg,while the recoveries and the coefficient of variation of fluoroquinolones were 77.5%-86.3% and 2.8%-7.4%,respectively.This method can be used for the sensitive and accurate determination of multiresidue in chicken muscle.
中文关键词	鸡肌肉 ; 高效液相色谱 ; 四环素类药物 ; 氟喹诺酮类药物 ; 多残留
英文关键词	chicken muscle high-performance liquid chromatography(HPLC) tetracyclines fluoroquinolones multiresidue
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:3720868
来源机构	中国农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/224508
作者单位	中国农业大学,动物医学院, 北京 100193, 中国
推荐引用方式 GB/T 7714	郭黎明,朱奎,丁双阳,等. 鸡肌肉组织中四环素类和氟喹诺酮类药物多残留检测[J]. 中国农业大学,2009,14(5):98-102.
APA	郭黎明,朱奎,丁双阳,江海洋,李建成,&李晓薇.(2009).鸡肌肉组织中四环素类和氟喹诺酮类药物多残留检测.,14(5),98-102.
MLA	郭黎明,et al."鸡肌肉组织中四环素类和氟喹诺酮类药物多残留检测".14.5(2009):98-102.