干旱区生态环境知识资源中心(Arid): 从塔里木兔体内首次分离出婴儿利什曼原虫

Arid

	从塔里木兔体内首次分离出婴儿利什曼原虫
其他题名	Leishmania infantum firstly isolated from Yarkend hare (Lepus yarkandensis)
	廖力夫 1; 燕顺生 1; 乌守巴特 1; 吴敏 2; 徐兵 1; 张勇 2; 侯岩岩 1; 雷刚 1
ISSN	1003-4692
出版年	2009
卷号	20 期号:1 页码:45-47
中文摘要	目的调查塔里木盆地荒漠型黑热病的保存宿主.方法开展动物与传播媒介及其环境的调查.冬季采集动物样品,用ELISA筛选抗体阳性动物;接种草原兔尾鼠和液体培养基培养分离利什曼原虫;分子生物学方法测定从当地黑热病患者、动物和传播媒介分离出的利什曼原虫特定基因序列.结果塔里木兔和家犬有抗利什曼原虫抗体;从44只抗体阳性塔里木兔中分离出3株利什曼原虫;塔里木兔、黑热病患者和吴氏白蛉分离出的利什曼原虫测定的NAGT核基因序列相同,与GenBank注册的婴儿利什曼原虫AF205934一致.结论塔里木兔是塔里木盆地荒漠型黑热病的野生宿主之一.
英文摘要	Objective To survey reservoir hosts of desert-type kala-azar in Tarim Basin. Methods Collect animal samples in winter, and screen the samples which antibody were positive by EL1SA. Leishmania was isolated by Lagurus lagurus inoculation and tissue culture. The specific gene sequences of Leishmania infantum isolated from kala-azar patients, Yarkend hare and Phlebotomus wui were amplified by molecular biological technology. Results There was Leishmania antibodies in Yarkend hare and Canis familiaris, and 3 strains of Leishmania were isolated from 44 of Yarkend hare which antibodies were positive. The NAGT gene sequences amplified from hares (3 strains), patients (1 strain) and vectors (6 strains) were same, according with the sequence of Linfantum (AF205934) from GenBank. Conclusion It suggests that yarkend hare is one of the primal host of desert-type kala-azar in Tarim Basin.
中文关键词	婴儿利什曼原虫 ; 荒漠型黑热病 ; 塔里木兔 ; 吴氏白蛉 ; 宿主
英文关键词	Leishmania infantum Kala-azar Lepus yarkandensis Phlebotomus wui Host
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:3572400
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/224506
作者单位	1.新疆维吾尔自治区疾病预防控制中心, 乌鲁木齐, 新疆 830002, 中国; 2.浙江大学生命科学学院, 杭州, 浙江中国
推荐引用方式 GB/T 7714	廖力夫,燕顺生,乌守巴特,等. 从塔里木兔体内首次分离出婴儿利什曼原虫[J],2009,20(1):45-47.
APA	廖力夫.,燕顺生.,乌守巴特.,吴敏.,徐兵.,...&雷刚.(2009).从塔里木兔体内首次分离出婴儿利什曼原虫.,20(1),45-47.
MLA	廖力夫,et al."从塔里木兔体内首次分离出婴儿利什曼原虫".20.1(2009):45-47.