干旱区生态环境知识资源中心(Arid): 高效液相色谱法对玉米中玉米赤霉烯酮的测定

Arid

	高效液相色谱法对玉米中玉米赤霉烯酮的测定
其他题名	Determination of Zearalenone in Maize by HPLC- FLD
	尹青岗; 王锋; 周洪杰; 张海伟; 谢芳; 哈益明
ISSN	1003-0174
出版年	2009
卷号	24 期号:7 页码:138-141
中文摘要	研究了高效液相色谱法测定玉米中玉米赤霉烯酮的方法.样品借鉴了GB/T 19540-2004中提取玉米赤霉烯酮的方法,通过Oasis HLB净化柱对提取液净化,以agilent extent C_(18)色谱柱为分离柱,乙腈-水(V_水:V_(乙腈)=55:45)为流动相进行荧光检测(lambdaex=235 nm,lambdaem=460 nm).玉米赤霉烯酮的质量浓度在12～2 400mu/kg范围内呈良好线性,相关系数为0.9994,对添加高、中、低3个浓度玉米赤霉烯酮的玉米样品进行加标回收试验,平均回收率分别为96.736%、93.839%、86.240%,变异系数在1%～10%之间,最低检测限为10mu/kg.此方法对玉米中玉米赤霉烯酮的检测是可行的,且可给谷物中玉米赤霉烯酮检测方法优化提供参考.
英文摘要	A method was established for determination of mycotoxin zearalenone(ZEN) in maize by high performance liquid chromatography(HPLC). Using the method of GB/T 19540:2004 as reference, samples were cleaned up through Oasis HLB column. Extracted samples were analyzed by HPLC on an agilent extent C18 column with acetonitrile/water (45 :55) as mobile phase (1.0 mL min 1 ), and fluorescence detection (lambdaex = 235 urn, lambdaem = 460 nm) was conducted. Re-salts: The calibration graphs are linear up from 12～2 400 mug/kg for ZEN in maize and correlation coefficient is 0.9994.For maize samples added with high, middle or low level of ZEN reference material, the recovery is 86.240%, 93. 839%,96.736%, respectively. The relative standard deviations are 1% ～ 10%, and the minimum detection limit is 10 mug/kg.This determination method for ZEN is feasible and may also provide reference to improve ZEN determination in other cereal grains.
中文关键词	玉米赤霉烯酮 ; 高效液相色谱 ; 荧光检测器 ; 玉米
英文关键词	zearalenone HPLC FLD maize
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PUBLIC ENVIRONMENTAL OCCUPATIONAL HEALTH
WOS研究方向	Public, Environmental & Occupational Health
CSCD记录号	CSCD:3635204
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/224505
作者单位	中国农业科学院农产品加工研究所, 北京 100193, 中国
推荐引用方式 GB/T 7714	尹青岗,王锋,周洪杰,等. 高效液相色谱法对玉米中玉米赤霉烯酮的测定[J],2009,24(7):138-141.
APA	尹青岗,王锋,周洪杰,张海伟,谢芳,&哈益明.(2009).高效液相色谱法对玉米中玉米赤霉烯酮的测定.,24(7),138-141.
MLA	尹青岗,et al."高效液相色谱法对玉米中玉米赤霉烯酮的测定".24.7(2009):138-141.