干旱区生态环境知识资源中心(Arid): SPE净化HPLC-APCI(+)-MS/MS分析肉类食品中硝基咪唑类药物原药及代谢物残留量

Arid

	SPE净化HPLC-APCI(+)-MS/MS分析肉类食品中硝基咪唑类药物原药及代谢物残留量
其他题名	Determination of Nitroimidazoles Drug Residues in Meat Products by HPLC-APCI(+)-MS/MS
	殷居易; 谢东华; 陈杰; 章再婷
ISSN	1004-2997
出版年	2009
卷号	30 期号:4 页码:193-200
中文摘要	采取固相萃取柱净化处理后进行液相色谱-大气压化学电离源串联四极杆质谱,在正离子检测模式下(HPLC-APCI(+)-MS/MS)对动物源性肉类食品中甲硝唑、地美硝唑(二甲硝唑)、替硝唑、洛硝唑(罗硝唑)、特尼哒唑、异丙硝唑、以及羟基化甲硝唑、羟基化异丙硝唑、2-羟甲基-1-甲基化-5-硝咪唑9种硝基咪唑类药物残留量进行分析.样品添加氘代标示物HMMNI-D~3、IPZ-OH-D~3后,用乙腈提取,通过OASIS MCX C_(18) SPE柱净化,Superiorex ODS C_(18) 色谱柱分离,采用梯度洗脱,流动相为0.1%甲酸水溶液和0.1%甲酸乙腈溶液,大气压电离源正离子MRM模式检测.肉类肌肉样品的方法定量下限(LOQ,S/N＞10)为0.5 mug·kg ~(-1) .在质量浓度0.5～100.0 mug·L~(-1) 范围内,峰面积与浓度呈良好的线性关系( r : 0.997 1～0.999 6).
英文摘要	An HPLC-APCI(+)MS-MS methodology was developed coupled with solid phase extraction decontamination for the residue determination of nitroimidazoles:metronidazole(MNZ),dimetridazole(DMZ),tindazole(TNZ),ronidazole(RNZ),ternidazole(TerNDZ),ipronidazole(IPZ),hydroxy-metronidazole(MNZOH),hydroxy-ipronidazole(IPZOH),2-hydroxymethyl-1-methyl-5-nitro-imidazole(HMMNI).The sample was extracted with the acetonitrile followed by appending deuterium-marker(HMMNI-D~3,IPZ-OH-D~3)internal standard,and operated for decontamination by OASIS MCX C_(18) cartridges.The chromatographic column was Superiorex ODS C_(18),the mobile phase was gradient solvent programming elution,which was consisted of water-acetic acid(0.1%)and acetonitrile-acetic acid(0.1%).Samples were ionized in flowing with a APCI ion source in positive mode,and were detected with MRM mode.The LOQ(S/N>10)of nitroimidazoles residues in meat sample is 0.5 mug·kg~(-1).The response for nitroimidazoles is linear in the range of 0.5-100.0 mug·L~(-1),and the correlation coefficient is excellent(r:0.997 1-0.999 6).
中文关键词	高效液相色谱串联四极杆质谱 ; 大气压电离源 ; 硝基咪唑类药物 ; 肉类食品 ; 柱净化
英文关键词	HPLC-MS/MS APCI nitroimidazoles drug meat products solid phase extraction
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3707689
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/224477
作者单位	宁波出入境检验检疫局技术中心,食品安全分析中心, 宁波, 浙江 315012, 中国
推荐引用方式 GB/T 7714	殷居易,谢东华,陈杰,等. SPE净化HPLC-APCI(+)-MS/MS分析肉类食品中硝基咪唑类药物原药及代谢物残留量[J],2009,30(4):193-200.
APA	殷居易,谢东华,陈杰,&章再婷.(2009).SPE净化HPLC-APCI(+)-MS/MS分析肉类食品中硝基咪唑类药物原药及代谢物残留量.,30(4),193-200.
MLA	殷居易,et al."SPE净化HPLC-APCI(+)-MS/MS分析肉类食品中硝基咪唑类药物原药及代谢物残留量".30.4(2009):193-200.