干旱区生态环境知识资源中心(Arid): 固相萃取HPLC/DAD法测定大鼠尿样中的延胡索乙素

Arid

	固相萃取HPLC/DAD法测定大鼠尿样中的延胡索乙素
其他题名	HPLC/DAD determination of tetrahydropalmatine with SPE in rat urine using solid phase extraction
	洪战英; 郑振; 闻俊; 范国荣; 柴逸峰; 吴玉田
ISSN	0254-1793
出版年	2009
卷号	29 期号:7 页码:1067-1069
中文摘要	目的:建立能同时分离分析大鼠尿样中延胡索乙素(THP)及其代谢产物的HPLC/DAD方法.方法:尿样经Oasis HLB C_(18)固相萃取小柱提取,采用HPLC/DAD法检测,色谱柱:Ultimate XB-C_(18)(4.6 mm*200 mm,5mum);流动相:A相为0.1%醋酸水溶液(三乙胺调节pH 5.5),B相为乙腈,梯度洗脱;流速为1.0 mL·min~(-1);检测波长230 nm;大鼠给药THP后,收集其尿样分析.结果:THP在0.10～50.0 mug·mL~(-1)的范围内有良好的线性关系(r=0.9999),最低定量限为0.10 mug·mL~(-1),平均方法回收率为99.5%,提取回收率为73.6%;日内和日间RSD小于9%.结论:所建立的方法准确可靠,可用于THP尿样浓度测定及其排泄动力学研究.
英文摘要	Objective: To develop an SPE - HPLC method for the determination of tetrahydropalmatine in rat urine. Methods:Samples were purified by Oasis HLB C_(18) SPE column. Separation was performed on Ultimate XB - C_(18) column (4. 6 mm*200 mm, 5mum) ,and the mobile phase was comprised of 0. 1% acetic acid solution (adjusted to pH 5.5 with triethylamine) - acetonitrile and delivered in gradient procedure. Flow rate was 1.0 mL·min~(-1) and the detection wavelength was set at 230 nm. Results: The calibration curve was linear in the range of 0. 10 -50. 0 mug· mL~(-1) for tetrahydropalmatine(r =0.9999) ,with LLOQ of 0. 10 mug·mL~(-1). The average recovery was 99. 5% and the extraction recovery was 73.6%. Intra - day and inter - day RSDs were less than 9%. Conclusion: The method is accurate, reliable and suitable for excretion study of THP in rat.
中文关键词	延胡索乙素 ; 固相萃取 ; 药物排泄
英文关键词	HPLC/DAD tetrahydropalmatine solid phase extraction (SPE) HPLC/DAD excretion
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PHARMACOLOGY PHARMACY
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:3511599
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/224405
作者单位	中国人民解放军第二军医大学药学院, 上海市药物代谢产物研究重点实验室, 上海 200433, 中国
推荐引用方式 GB/T 7714	洪战英,郑振,闻俊,等. 固相萃取HPLC/DAD法测定大鼠尿样中的延胡索乙素[J],2009,29(7):1067-1069.
APA	洪战英,郑振,闻俊,范国荣,柴逸峰,&吴玉田.(2009).固相萃取HPLC/DAD法测定大鼠尿样中的延胡索乙素.,29(7),1067-1069.
MLA	洪战英,et al."固相萃取HPLC/DAD法测定大鼠尿样中的延胡索乙素".29.7(2009):1067-1069.