干旱区生态环境知识资源中心(Arid): 固相萃取-高效液相色谱法测定水果和果酱中的6种对羟基苯甲酸酯

Arid

	固相萃取-高效液相色谱法测定水果和果酱中的6种对羟基苯甲酸酯
其他题名	Determination of six p-hydroxybenzoates in fruits and jams using solid-phase extraction-high performance liquid chromatography
	陈皑 1; 何乔桑 2; 王萍亚 1; 周勇 1; 黄鹂 1; 许镇坚 1; 赵华 1; 张薇英 1
ISSN	1000-8713
出版年	2009
卷号	27 期号:6 页码:804-808
中文摘要	建立了固相萃取-高效液相色谱(SPE-HPLC)同时测定水果和果酱中6种对羟基苯甲酸酯(对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸异丙酯、对羟基苯甲酸丙酯、对羟基苯甲酸异丁酯和对羟基苯甲酸丁酯)含量的方法.经Oasis HLB固相萃取柱净化的样品采用HPLC分离,优化的色谱条件为采用Symmetry-C_(18)色谱柱分离,流动相为柠檬酸缓冲液-甲醇(体积比为48∶52),流速1.0 mL/min,检测波长258 nm,测定温度40 ℃.6种对羟基苯甲酸酯的线性范围为0.1～20.0 mg/L(r=0.999 9),回收率为82.8% ～115.5%,相对标准偏差为0.2% ～6.8%(n=6).对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸异丙酯、对羟基苯甲酸丙酯的检出限(S/N=3)为0.1 mg/kg,定量限(S/N=10)为0.3 mg/kg;对羟基苯甲酸异丁酯和对羟基苯甲酸丁酯的检出限为0.2 mg/kg,定量限为0.6 mg/kg.该方法简便快速、结果准确、重现性好,可作为测定水果及果酱中多种对羟基苯甲酸酯的有效方法.
英文摘要	A method was developed for the determination of 6 p-hydroxybenzoates (methyl p-hydroxybenzoate ( MHB ) , ethyl p-hydroxybenzoate ( EHB ), isopropyl p-hydroxybenzoate (IPHB), propyl p-hydroxybenzoate (PHB), isobutyl p-hydroxybenzoate (IBHB) and butyl p-hydroxybenzoate (BHB)) in fruits and jams using the combination of solid-phase extraction and high performance liquid chromatography (SPE-HPLC). Two different extraction solutions and three different mobile phases were tested for p-hydroxybenzoates analysis, and finally etha-nol was used as the extraction solvent and methanol-citric acid buffer was selected as the mo-bile phase. The sample was extracted, and purified by an Oasis HLB solid-phase extraction car-tridge, then separated on a Symmetry-C_(18) column and detected at the wave length of 258 nm. The results showed that all the calibration graphs were linear in the concentration range of 0. 1 - 20. 0 mg/L (r = 0. 9999). The detection limits and quantification limits were 0. 1 mg/kg (S/N = 3) and 0. 3 mg/kg (S/N =10) respectively for MHB, EHB, IPHB and PHB, 0. 2 mg/kg (S/N = 3) and 0. 6 mg/kg (S/N = 10) respectively for IBHB and BHB. The average recoveries were between 82. 8% and 115. 5% with the relative standard deviations (RSDs) of 0. 2% -6. 8% (n = 6). The method is simple, rapid, sensitive and reproducible, and can be used for the routine analysis of the p-hydroxybenzoates in fruits and jams.
中文关键词	固相萃取 ; 高效液相色谱法 ; 对羟基苯甲酸酯 ; 水果 ; 果酱
英文关键词	solid phase extraction (SPE) high performance liquid chromatography (HPLC) p-hydroxybenzoates fruits jams
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3716220
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/223996
作者单位	1.国家海洋食品质量监督检验中心, 舟山, 浙江 316021, 中国; 2.浙江省食品质量安全检测院, 杭州, 浙江 310013, 中国
推荐引用方式 GB/T 7714	陈皑,何乔桑,王萍亚,等. 固相萃取-高效液相色谱法测定水果和果酱中的6种对羟基苯甲酸酯[J],2009,27(6):804-808.
APA	陈皑.,何乔桑.,王萍亚.,周勇.,黄鹂.,...&张薇英.(2009).固相萃取-高效液相色谱法测定水果和果酱中的6种对羟基苯甲酸酯.,27(6),804-808.
MLA	陈皑,et al."固相萃取-高效液相色谱法测定水果和果酱中的6种对羟基苯甲酸酯".27.6(2009):804-808.