干旱区生态环境知识资源中心(Arid): Desert Hedgehog诱导大鼠胚胎中脑神经前体细胞定向分化为多巴胺能神经元

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	Desert Hedgehog诱导大鼠胚胎中脑神经前体细胞定向分化为多巴胺能神经元
其他题名	Desert Hedgehog induces the differentiation of neural progenitor cells from embryonic mesencephalon of rat into dopaminergic neurons
	杨彩侠 1; 赵焕英 2; 赵春礼 2; 刘靖 3; 徐群渊 2; 高福禄 4
ISSN	1001-6325
出版年	2009
卷号	29 期号:4 页码:360-365
中文摘要	目的研究Desert Hedgehog(DHH)对胚胎中脑神经前体细胞(NPCs)向多巴胺能神经元分化的诱导作用.方法大鼠DHH病毒上清感染COS7、NIH/3T3和9L细胞,用免疫荧光、实时定量PCR和Western blot方法检测DHH表达.分离培养大鼠胚胎中脑神经前体细胞,免疫荧光鉴定神经前体细胞的增殖分化特性.条件细胞表达DHH成功后分别与上述NPCs共培养10 d,免疫荧光检测酪氨酸羟化酶(TH)的表达.结果 DHH在COS7、NIH/313和9L细胞中成功表达;同时,分离培养的大鼠胚胎中脑神经前体细胞具有良好的增殖分化特性,但两者共培养10 d后偶见TH阳性的细胞.结论 DHH编码的蛋白不能或不能单独诱导NPCs定向分化为多巴胺能神经元.
英文摘要	Objective To investigate the effect of Desert Hedgehog on direct differentiation of neural progenitor cells (NPCs) cultured from embryonic mesencephalon in the rats. Methods We infected DHH into C0S7, NIH/ 3T3 and 9L cells, and detected the expression of DHH in the cells with immunofluorescence, real-time PCR and Western blot. All of the three cells were co-cultured with NPCs isolated from ventral mesencephalon in embryonic SD rats (E13. 5) respectively. Immunoreactivities of tyrosine hydroxylase (TH) was detected by immunocytochem-istry after 10 days. Results The expression of DHH in C0S7, NIH/3T3 and 9L cells was remarkably detected, but few TH-positive cells were found in the three co-cultral systems at the 10th day. Conclusion The protein derived from DHH itself does not show any inductive effect on the differentiation of NPCs to the dopaminergic neurons in vitro.
中文关键词	神经前体细胞 ; 多巴胺能神经元 ; 分化 ; 大鼠
英文关键词	Desert Hedgehog Desert Hedgehog neural progenitor cell dopaminergic neuron differentiation rat
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:3517416
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/223857
作者单位	1.河北医科大学,组织学与胚胎学教研室, 石家庄, 河北 050017, 中国; 2.首都医科大学,北京神经科学研究所, 北京 100069, 中国; 3.医学论坛杂志, 北京 100069, 中国; 4.河北师范大学, 石家庄, 河北 050017, 中国
推荐引用方式 GB/T 7714	杨彩侠,赵焕英,赵春礼,等. Desert Hedgehog诱导大鼠胚胎中脑神经前体细胞定向分化为多巴胺能神经元[J],2009,29(4):360-365.
APA	杨彩侠,赵焕英,赵春礼,刘靖,徐群渊,&高福禄.(2009).Desert Hedgehog诱导大鼠胚胎中脑神经前体细胞定向分化为多巴胺能神经元.,29(4),360-365.
MLA	杨彩侠,et al."Desert Hedgehog诱导大鼠胚胎中脑神经前体细胞定向分化为多巴胺能神经元".29.4(2009):360-365.