干旱区生态环境知识资源中心(Arid): 食品中三聚氰胺的固相萃取-高效液相色谱测定法

Arid

	食品中三聚氰胺的固相萃取-高效液相色谱测定法
其他题名	Determination of Melamine in Foods by Solid Phase Extraction-High Performance Liquid Chromatography
	李建平; 夏义平; 张明月; 渠志华; 吕光
ISSN	1001-5914
出版年	2009
卷号	26 期号:7 页码:635-636
中文摘要	目的建立固相萃取(SPE)-高效液相色谱(HPLC)测定食品中三聚氰胺含量的方法.方法样品经提取液提取、离心后,上清液于Waters Oasis MCX固相萃取柱净化.采用HPLC进行测定,色谱柱为TIANHE C18柱(250 mm*4.6 mm,5mum),流动相为混合溶液(含0.01 mol/L己烷磺酸钠和0.01 mol/L柠檬酸):乙腈体积比为94:6,流量为1.0 ml/min,柱温为35℃,进样量为20muL.结果在0.80-80.0mg/L范围内,该方法所得线性方程为:y=84.64x-6.1271,相关系数为0.99998,检出限为0.5mg/kg,平均加标回收率为88.8%～99.0%,RSD＜5%.结论该方法操作简便、快速,适用于各类食品中三聚氰胺含量的检测.
英文摘要	Objective To establish a solid phase extraction (SPE)-high performance liquid chromatography(HPLC) method for determination of melamine in foods. Methods Melamine in sample was extracted with extractive agent. After centrifugation, the supernatant fluid was purified by Waters Oasis MCX cartridge, and detected with HPLC method. Chromatographic column of TIANHE C_(18)(250 mm*4.6 mm,5 mum) was used at 35 ℃ and mobile phase was water(containing 0.01 mol/L 1-hexanesulfonic acid sodium salt and 0.01 mol/L citric acid): acetonitrile = 94:6 (V:V) at a flow rate of 1.0 ml/min. Injection volume was 20 ui. Results In the range of 0.80 to 80.0 mg/L, the regression equation was y=84.64 x-6.127 1 with the correlation coefficient of 0.999 98. The detection limit of melamine in foods was 0.5 mg/kg. The average rates of recovery were 88.0%-96.0% and the relative standard deviation was less than 5%. Conclusion The method established in the present paper is applicable to determination of melamine in foods with good precision,accuracy and sensitivity.
中文关键词	色谱法 ; 液相 ; 三聚氰胺 ; 食品 ; 固相萃取
英文关键词	Chromatography liquid Melamine Foods Solid phase extraction
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3715767
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/223856
作者单位	天津市疾病预防控制中心理化室, 天津 300011, 中国
推荐引用方式 GB/T 7714	李建平,夏义平,张明月,等. 食品中三聚氰胺的固相萃取-高效液相色谱测定法[J],2009,26(7):635-636.
APA	李建平,夏义平,张明月,渠志华,&吕光.(2009).食品中三聚氰胺的固相萃取-高效液相色谱测定法.,26(7),635-636.
MLA	李建平,et al."食品中三聚氰胺的固相萃取-高效液相色谱测定法".26.7(2009):635-636.