干旱区生态环境知识资源中心(Arid): 高效液相色谱-电喷雾串联质谱法测定鳗鱼中三聚氰胺的残留

Arid

	高效液相色谱-电喷雾串联质谱法测定鳗鱼中三聚氰胺的残留
其他题名	Determination of melamine residue in eel using high performance liquid chromatography-electrospray ionization tandem mass spectrometry
	邢丽红 1; 冷凯良 1; 孙伟红 1; 谭志军 1; 苗钧魁 1; 王泽文 2
ISSN	1000-3096
出版年	2009
卷号	33 期号:8 页码:1-4,53
中文摘要	建立了利用高效液相色谱-电喷雾串联质谱(LC-MS/MS)法测定鳗鱼(Anguilla japonica)中三聚氰胺残留的方法.采用乙腈和三氯乙酸溶液沉淀蛋白,MCX固相萃取柱净化,提取目标分析物,经SCX-C18均匀混合填装的反相色谱柱分离,选择反应监测(SRM)正离子模式测定.三聚氰胺的定量限为20mug/kg,在20～500mug/kg时,峰强度与质量浓度的线性关系良好(r＞0.99).方法的平均回收率为84.82%～91.42%.
英文摘要	The method for the determination of melamine residue in eel using high performance liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS) was developed. Acetonitril and 1 % trichloracetic acid solution were used to precipitate proteins and to dissociate the target analyte from the sample matrix. Then it was degrased and depurated with Oasis MCX solid phase extraction cartridge. A SCX-C18 mixture filled RP-HPLC column was used for separation, and the mass spectrometer was operated in the positive ion mode using select reaction monitoring. The limit of detection was 20 mu/kg for LC-MS/MS. The correlation coefficient of linear calibration curve was over 0. 99 within the melamine concentration range 20～500 mu/kg. The average recovery for melamine ranged between 84. 82% and 91. 42%.
中文关键词	鳗鱼(Anguilla japonica) ; 三聚氰胺 ; 液相色谱串联质谱仪 ; 残留 ; 测定
英文关键词	eel melamine high performance liquid chromatography-electrospray ionization tandem mass spectrometry residue determination
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FISHERIES
WOS研究方向	Fisheries
CSCD记录号	CSCD:3746176
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/223805
作者单位	1.中国水产科学研究院,黄海水产研究所, 青岛, 山东 266071, 中国; 2.中国海洋大学, 青岛, 山东 266003, 中国
推荐引用方式 GB/T 7714	邢丽红,冷凯良,孙伟红,等. 高效液相色谱-电喷雾串联质谱法测定鳗鱼中三聚氰胺的残留[J],2009,33(8):1-4,53.
APA	邢丽红,冷凯良,孙伟红,谭志军,苗钧魁,&王泽文.(2009).高效液相色谱-电喷雾串联质谱法测定鳗鱼中三聚氰胺的残留.,33(8),1-4,53.
MLA	邢丽红,et al."高效液相色谱-电喷雾串联质谱法测定鳗鱼中三聚氰胺的残留".33.8(2009):1-4,53.