干旱区生态环境知识资源中心(Arid): 高效液相色谱-串联质谱法检测花生中的黄曲霉毒素B

Arid

	高效液相色谱-串联质谱法检测花生中的黄曲霉毒素B_1
其他题名	Determination of aflatoxin B_1 in peanuts by liquid chromatography-tandem mass spectrometry
	杨静; 哈益明; 王锋
ISSN	1000-0720
出版年	2009
卷号	28 期号:6 页码:35-38
中文摘要	应用高效液相色谱-电喷雾串联四极杆质谱联用系统(HPLC-MS/MS),在多反应离子检测方式(MRM)下,对花生中的黄曲霉毒素B1进行检测.对花生中黄曲霉毒素B_1的提取、净化、液相分离及串联质谱等相关检测参数进行了优化研究.用V(甲醇):V(水)=6:4提取,OASIS HLB SPE小柱净化,定容过滤.采用V(甲醇):V(水)(含体积分数0.1%甲酸)=7:3为流动相,前级离子313.0,二级离子241.1、269.1,ESI正离子方式检测,在3.3 min出峰.结果表明,在ESI正离子模式下,黄曲霉毒素B_1在其线性定量范围0.1～50mug/kg内,相关系数达到0.9999,检出限为0.03mug/kg,最低定量限为0.1mug/kg.低、中、高浓度添加回收率范围为93%～105%.
英文摘要	An analytical method for the determination of aflatoxin B_1 in peanuts by high performance liquid chromatog-raphy-tandem mass spectrometry has been developed. This article focused on the optimization of extraction, clean-up, HPLC separation and MS/MS parameters of the analyte. Aflatoxin B_1 was extracted by 60% ( V/V) of methanol aqueous solution, and then it was purified by OASIS HLB SPE column. The eluted extract was analyzed by HPLC-MS/MS in positive ion mode using multiple reaction monitoring with a triple-quadruple mass spectrometer using an electrospray ionization source. An isocratic mobile phase composed of methanol-water (70/30) (V/V) was used, water with 0.1% formic acid (V/V) . With the precursor ion =313.0 and the product ion = 241. 1、269.1, the retention time of aflatoxin B_1 was 3.30 min. Under the ESI + analysis, a high correlation coefficients ( > 0.999) of aflatoxin B1 was obtained within linear range (0.1 ～ 50 mug/kg). Sample recoveries at three spiking levels ranged from 93% to 105% (relative standard deviation (RSD<5%). Determination limit (S/N = 3) was 0.03 mug/kg and quantification limit (S/N=10) was 0.1 mug/kg.
中文关键词	黄曲霉毒素B_1 ; 高效液相色谱串联质谱 ; 多离子反应检测
英文关键词	Aflatoxin B_1 HPLC-MS/MS Multiple reaction monitoring
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3577008
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/223490
作者单位	中国农业科学院农产品加工研究所, 北京 100193, 中国
推荐引用方式 GB/T 7714	杨静,哈益明,王锋. 高效液相色谱-串联质谱法检测花生中的黄曲霉毒素B_1[J],2009,28(6):35-38.
APA	杨静,哈益明,&王锋.(2009).高效液相色谱-串联质谱法检测花生中的黄曲霉毒素B_1.,28(6),35-38.
MLA	杨静,et al."高效液相色谱-串联质谱法检测花生中的黄曲霉毒素B_1".28.6(2009):35-38.