干旱区生态环境知识资源中心(Arid): 高效液相色谱法测定动物组织中3-甲基喹喔啉-2-羧酸残留量

Arid

	高效液相色谱法测定动物组织中3-甲基喹喔啉-2-羧酸残留量
其他题名	Determination of the Marker Residue of Olaquindox, Methyl-3-quinoxaline-2-carboxylic Acid in Animal Edible Tissues Using High Performance Liquid Chromatography
	吴玉杰 1; 陈冬梅 2; 陶燕飞 2; 袁宗辉 2; 刘军义 1
ISSN	1006-6144
出版年	2009
卷号	25 期号:4 页码:427-430
中文摘要	本文建立了猪、鸡、鱼肌肉及猪、鸡肝脏组织中喹乙醇代谢残留标识物3-甲基喹喔啉-2-羧酸残留量的高效液相色谱分析方法.样品在酸性环境水解,经乙酸乙酯、磷酸盐缓冲溶液依次提取,Oasis MAX固相萃取柱净化,高效液相色谱紫外测定(HPLC-UV).3-甲基喹喔啉-2-羧酸标准曲线相关系数(r~2)为0.9992(10～1 000 mug/L);不同动物组织样品中3-甲基喹喔啉-2-羧酸工作曲线相关系数(r)为0.9995～0.9997(2～100 mug/kg).在2～100 mug/kg浓度范围,3-甲基喹喔啉-2-羧酸回收率为69%～104%,日内相对标准偏差＜15%,日间相对标准偏差＜20%.检出限(CCalpha)为1.2～4.3 mug/kg,定量限(CCbeta)为2.0～5.6 mug/kg.
英文摘要	A method was developed for the quantitative determination of methyl-3-quinoxaline-2-carb-oxylic acid(MQCA),the metabolite that has been designated as the marker residue for olaquindox in swine tissue.This is the first report on simultaneous determination of MQCA in pork,chicken,fish and porcine,chicken livers.Samples were deproteinated with 5% metaphosphoric acid in 10% methanol followed by liquid-liquid extraction.Further clean-up was performed by solid phase extraction(SPE) through mixed mode anion-exchange columns(Oasis MAX SPE).Analysis was performed with RP-C18 column HPLC coupled to UV detector.The method has good linearity ranged from 2 to 100 mug/kg in tissues with recoveries from 69% to 104%,inter-day RSDs less than 15% and intra-day RSDs less than 20%,and the detection limit(CCalpha)(1.2～4.3 mug/kg) and the quantitation limit(CCbeta)(2.0～5.6 mug/kg) have been obtained in different tissues.
中文关键词	喹乙醇 ; 3-甲基喹喔啉-2-羧酸 ; 固相萃取 ; 高效液相色谱法 ; 动物组织
英文关键词	Olaquindox Methyl-3-quinoxaline-2-carboxylic acid Oasis MAX SPE High performance liquid chromatography Animal tissues
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3656842
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/223488
作者单位	1.广西出入境检验检疫局, 南宁, 广西 530021, 中国; 2.华中农业大学, 国家兽药残留基准实验室, 武汉, 湖北 430070, 中国
推荐引用方式 GB/T 7714	吴玉杰,陈冬梅,陶燕飞,等. 高效液相色谱法测定动物组织中3-甲基喹喔啉-2-羧酸残留量[J],2009,25(4):427-430.
APA	吴玉杰,陈冬梅,陶燕飞,袁宗辉,&刘军义.(2009).高效液相色谱法测定动物组织中3-甲基喹喔啉-2-羧酸残留量.,25(4),427-430.
MLA	吴玉杰,et al."高效液相色谱法测定动物组织中3-甲基喹喔啉-2-羧酸残留量".25.4(2009):427-430.