干旱区生态环境知识资源中心(Arid): 固相萃取-亲水作用色谱法测定废水中四环素类抗生素

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	固相萃取-亲水作用色谱法测定废水中四环素类抗生素
其他题名	Solid Phase Extraction-Hydrophilic Interaction Chromatographic Determination of Tetracycline Antibiotics in Wastewater
	张艺; 李瑞萍; 张兴华; 喻玺; 黄应平
ISSN	0253-3820
出版年	2009
卷号	37 期号:6 页码:840-844
中文摘要	建立了固相萃取(SPE)-亲水作用色谱法(HILIC)测定废水中金霉素(CTC)、强力霉素(DC)、四环素(TC)和土霉素(OTC)4种四环素类抗生素(TCs)残留的新方法.水样经Oasis HLB固相萃取柱净化富集后, 采用以氨基色谱柱及高极性有机溶剂-水相缓冲溶液为流动相的亲水作用色谱法(HILIC)进行分析. 对流动相中缓冲溶液的类型和pH值、离子强度、有机溶剂的浓度以及流速进行了优化, 确定了以V(乙腈)∶V(6.7mmol/L柠檬酸铵缓冲溶液, pH 4.0)=85∶15混合液为流动相的最佳条件.本方法具有良好的线性关系(r>0.999)和重现性(峰面积RSD<1.0%),最低检出限(S/N=3)为12～30mug/L, 4种四环素类药物添加水平在0.5～10mug/L范围内的标准加入回收率为 90.6%～106.5%;相对标准偏差为2.5%～6.2%.本方法简便、准确、流动相离子强度低,适合于与质谱联用,用于屠宰场污水及医院污水等实际样品检测,结果满意.
英文摘要	A novel method for the determination of tetracycline antibiotics (TCs),i.e.oxytetracycline (OTC), tetracycline (TC), chlortetracycline(CTC) and doxycycline (DC) in wastewater sample by hydrophilic interaction chromatography(HILIC)using amino-silica column with highly polar organic-aqueous buffer as mobile phase has been developed. TCs were collected and purified from wastewater using Oasis HLB cartridges. Separation parameters such as the buffer pH,buffer ionic strength, organic modifier concentration and flow rate have been optimized. The optimal mobile phase was 85% (V/V) acetonitrile in 6. 7 mmol/L ammonium citrate buffer(pH 4.0).The developed method had good linearity(r>0.999) and reproducibility (RSD <1.0% for the peak area). The limits of detection for the TCs tested were between 12-30 mug/L (S/N=3).Recoveries of TCs at the concentration of 0.5-10mug/L ranged from 90.6% to 106.5% with RSD 2.5%-6.2%.The proposed method is simple, accurate and the mobile phase is compatible for mass spectrometry due to its low ionic strength. It has been applied to the determination of TCs in abattoir wastewater samples with satisfactory results.
中文关键词	亲水作用色谱 ; 四环素类抗生素 ; 固相萃取 ; 废水
英文关键词	Hydrophilic interaction chromatography tetracycline antibiotics solid phase extraction wastewater samples
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY MULTIDISCIPLINARY
WOS研究方向	Chemistry
CSCD记录号	CSCD:3576886
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/223487
作者单位	三峡大学Alan G. MacDiarmid再生能源研究所, 宜昌, 湖北 443002, 中国
推荐引用方式 GB/T 7714	张艺,李瑞萍,张兴华,等. 固相萃取-亲水作用色谱法测定废水中四环素类抗生素[J],2009,37(6):840-844.
APA	张艺,李瑞萍,张兴华,喻玺,&黄应平.(2009).固相萃取-亲水作用色谱法测定废水中四环素类抗生素.,37(6),840-844.
MLA	张艺,et al."固相萃取-亲水作用色谱法测定废水中四环素类抗生素".37.6(2009):840-844.