干旱区生态环境知识资源中心(Arid): 高效液相色谱法快速检测猪组织中泰妙菌素残留

Arid

	高效液相色谱法快速检测猪组织中泰妙菌素残留
其他题名	A Rapid High Performance Liquid Chromatographic Method for Determination of Tiamulin Residue in Swine Tissues
	程林丽; 张素霞; 沈建忠; 王战辉; 李彦伸; 冯培生
ISSN	0253-3820
出版年	2009
卷号	37 期号:5 页码:718-720
中文摘要	应用高效液相色谱法测定猪组织中泰妙菌素残留.试样经磷酸盐缓冲液(pH 8.0)-乙酸乙酯混合提取,离心后加0.1%酒石酸,40 ℃旋转蒸发,除尽有机相,加正已烷除脂,HLB柱净化后,氮气吹干,0.0125%甲酸溶解后HPLC检测.流动相为乙腈-0.0125%甲酸(42∶ 58,V/V).紫外检测波长为210 nm.对猪肉(猪肝)进行15(25)～500 mug/kg的药物添加回收实验,结果表明,泰妙菌素的平均回收率为80.1%～92.7%; 日内相对标准偏差为2.0%～10.2%;日间相对标准偏差为5.9%～13.9%.按信噪比S/N=3计算,猪肉和猪肝中泰妙菌素的检出限(LOD)分别为4.5和7.5 mug/kg.
英文摘要	A rapid method has been developed for the determination of tiamulin (TML) residue in swine tissues by high performance liquid chromatography. The samples were extracted with phosphate buffer-ethyl acetate solution, and then centrifuged by 5000 rpm for 10 min, 0.1% fartrate solution was added in the supernatant obtained with liguid-liquid-liquid extraction, and the organic phase was eveporated. After fats having been removed with hexan, further purification was performed with Oasis HLB solid phase extraction column. The TML residue was determined by HPLC with a mobile phase of acetonitrile-0. 0125% acetic acid(42:58, V/V) and UV detection at 210 nm. The average recovery rates of TML in pork and swine liver were in the range of 80. 1% -92.7% when spiked 25 -500 mug/kg. The RSD ranged from 2.0% to 13.9%. The detection limits were 5 mug/kg and 8 mug/kg for pork and swine liver, respectively.
中文关键词	泰妙菌素 ; 猪肉 ; 猪肝 ; 高效液相色谱
英文关键词	Tiamulin pork swine liver high performance liquid chromatography
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY MULTIDISCIPLINARY
WOS研究方向	Chemistry
CSCD记录号	CSCD:3581943
来源机构	中国农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/223486
作者单位	中国农业大学动物医学院, 北京 100193, 中国
推荐引用方式 GB/T 7714	程林丽,张素霞,沈建忠,等. 高效液相色谱法快速检测猪组织中泰妙菌素残留[J]. 中国农业大学,2009,37(5):718-720.
APA	程林丽,张素霞,沈建忠,王战辉,李彦伸,&冯培生.(2009).高效液相色谱法快速检测猪组织中泰妙菌素残留.,37(5),718-720.
MLA	程林丽,et al."高效液相色谱法快速检测猪组织中泰妙菌素残留".37.5(2009):718-720.