干旱区生态环境知识资源中心(Arid): 超高效液相色谱-质谱对动物源食品中氯丙嗪、异丙嗪及其代谢物的测定

Arid

	超高效液相色谱-质谱对动物源食品中氯丙嗪、异丙嗪及其代谢物的测定
其他题名	Determination of Chlorpromazine and Promethazine and Their Metabolites in Animal Food Using Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry
	齐士林 1; 吴敏 2; 严丽娟 2; 吴抒怀 2; 邹伟 2; 林立毅 2; 周昱 2
ISSN	1004-4957
出版年	2009
卷号	28 期号:6 页码:677-681
中文摘要	建立了动物源食品中吩噻嗪类药物氯丙嗪、异丙嗪及其代谢物氯丙嗪亚砜和异丙嗪亚砜同时测定的固相萃取超高效液相色谱-电喷雾电离串联四极杆质谱(UPLC-ESI MS/MS)方法.样品用乙酸乙酯和氢氧化钠溶液提取,Oasis HLB柱富集净化,超高效液相色谱-电喷雾串联四极杆质谱仪检测,采用多反应监测正离子模式,可以一次性对动物源性食品中的吩噻嗪类药物氯丙嗪、异丙嗪及其代谢物氯丙嗪亚砜和异丙嗪亚砜进行定性和定量测定.方法在 1 ～100 mug/L范围具有良好的线性,回收率为76% ～96%,定量下限(S/N＞10)为异丙嗪5 mug/kg,氯丙嗪、氯丙嗪亚砜、异丙嗪亚砜1 mug/kg.检出限(S/N=3)为异丙嗪1.5 mug/kg,氯丙嗪、氯丙嗪亚砜、异丙嗪亚砜0.3 mug/kg.
英文摘要	A method for the simultaneous detection residues of chlorpromazine and promethazine and their metabolites chlorpromazine sulphoxide and promethazine sulphoxide in animal foods was developed by using solid phase extraction (SPE) combination with ultra performance liquid chromatography -electrospray ionization triple-quadruple tandem mass spectrometry(UPLC -ESI MS/MS). The chlorpromazine and promethazine and their metabolites in the test sample was extracted with ethyl acetate and sodium hydroxide solution. The homogenate was centrifuged and the supernatant was collected. The extract was cleaned up and concentrated on an Oasis HLB cartridge, and then analyzed by UPLC -ESI MS/MS under the positive ion mode using multiple reaction monitoring(MRM) mode. Extraction and chromatographic conditions were optimized and the best separation was obtained. The cabalibra-tion curves were linear in the range of 1 - 100 mug/L. The average recoveries were between 76% and 96% (n =6) in the spiked range of 5 - 100 mug/kg in pork, fish, dullfrog and milk. The limits of quantification(S/N> 10) were in the range of 1 -5mug/kg and the limits of detection(S/N = 3) were 0.3-1.5 mug/kg.
中文关键词	固相萃取 ; 超高效液相色谱-电喷雾电离质谱 ; 氯丙嗪 ; 异丙嗪 ; 氯丙嗪亚砜 ; 异丙嗪亚砜 ; 动物源性食品
英文关键词	solid phase extraction (SPE) ultra performance liquid chromatography -electrospray ionization triple-quadruple tandem mass spectrometry ( UPLC - ESI MS/MS ) chlorpromazine promethazine chlorpromazine sulphoxide promethazine sulphoxide animal
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3516837
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/223480
作者单位	1.厦门大学, 厦门, 福建 361002, 中国; 2.厦门出入境检验检疫局, 厦门, 福建 361026, 中国
推荐引用方式 GB/T 7714	齐士林,吴敏,严丽娟,等. 超高效液相色谱-质谱对动物源食品中氯丙嗪、异丙嗪及其代谢物的测定[J],2009,28(6):677-681.
APA	齐士林.,吴敏.,严丽娟.,吴抒怀.,邹伟.,...&周昱.(2009).超高效液相色谱-质谱对动物源食品中氯丙嗪、异丙嗪及其代谢物的测定.,28(6),677-681.
MLA	齐士林,et al."超高效液相色谱-质谱对动物源食品中氯丙嗪、异丙嗪及其代谢物的测定".28.6(2009):677-681.