干旱区生态环境知识资源中心(Arid): 动物组织与水产品中头孢氨苄残留量的液相色谱-串联质谱检测

Arid

	动物组织与水产品中头孢氨苄残留量的液相色谱-串联质谱检测
其他题名	Determination of Cefalexin Residue in Animal Tissue and Aquatic Products by LC-MS/MS
	倪梅林; 崔晓美; 殷居易; 彭锦峰; 房科腾; 曹苏仙; 谢东华
ISSN	1004-4957
出版年	2009
卷号	28 期号:4 页码:489-492
中文摘要	建立了牛肉、猪肉、肝脏、肾脏、脂肪、鱼肉、虾肉中头孢氨苄残留的LC-MS/MS检测方法.组织样品中的头孢氨苄用甲醇-0.2%偏磷酸 (体积比3 : 7)溶液提取,采用Oasis HLB固相萃取小柱净化.分析样品以甲酸溶液(体积分数0.1%)-乙腈为流动相,经MG-Ⅱ C_(18)色谱柱分离,在LC-MS/MS多反应监测模式下进行定性、定量分析,采用正离子扫描.头孢氨苄的定量下限为0.01 mg/kg,动物组织和水产品样品在0.01、0.05、0.1、0.2 mg/kg添加水平的回收率为75% ～106%,相对标准偏差(n=10)为5.3% ～12.1%.
英文摘要	A liquid chromatography - tandam mass spectrometric (LC-MS/MS) method was developed for the determination of cefalexin residue in beef, pork, liver, kidney, fat, fish and shrimp tissue sample. The sample was extracted by methanol-0.2% metaphosphoric acid with volume ratio of 3:7 and cleaned up by an Oasis HLB solid phase extraction (SPE) cartridge. The analyte was separated on an MG- Ⅱ C_(18) column using 0.1% formic acid - acetonitrile as mobile phase, and determined qualitatively and quantitatively by LC-MS/MS under multi-reaction monitoring(MRM) mode using positive ion scaning mode. The limit of quatitation( LOQ, S/N = 10) for cefalexin was 0.01 mg/kg. The recoveries of cefalexin from animal tissue and aquatic products spiked with four levels at 0.01, 0.05, 0.1, 0.2 mg/kg were in the range of 75% - 106% with the relative standard deviations of 5.3% -12.1%(n = 10).
中文关键词	头孢氨苄 ; 液相色谱-串联质谱 ; 残留 ; 动物组织 ; 水产品
英文关键词	cefalexin LC-MS/MS residue animal tissue aquatic products
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL ; AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Chemistry ; Agriculture
CSCD记录号	CSCD:3505070
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/223479
作者单位	宁波出入境检验检疫局, 宁波, 浙江 315012, 中国
推荐引用方式 GB/T 7714	倪梅林,崔晓美,殷居易,等. 动物组织与水产品中头孢氨苄残留量的液相色谱-串联质谱检测[J],2009,28(4):489-492.
APA	倪梅林.,崔晓美.,殷居易.,彭锦峰.,房科腾.,...&谢东华.(2009).动物组织与水产品中头孢氨苄残留量的液相色谱-串联质谱检测.,28(4),489-492.
MLA	倪梅林,et al."动物组织与水产品中头孢氨苄残留量的液相色谱-串联质谱检测".28.4(2009):489-492.