干旱区生态环境知识资源中心(Arid): 昆虫抗冻蛋白基因转化烟草的抗寒性

Arid

	昆虫抗冻蛋白基因转化烟草的抗寒性
其他题名	Cold Tolerance of Transgenic Tobacco Carrying Gene Encoding Insect Antifreeze Protein
	王艳; 邱立明; 谢文娟; 黄薇; 叶锋; 张富春; 马纪
ISSN	0496-3490
出版年	2008
卷号	34 期号:3 页码:397-402
中文摘要	将准噶尔小胸鳖甲抗冻蛋白基因MPAFP149亚克隆至pCAMBIA1302中,构建成单价植物表达载体pCAMBIA1302-MPAFP149,转化至农杆菌EHA105中,利用叶圆盘法转化烟草。通过PCR、PCR-Southern及RT-PCR检测表明,抗冻蛋白基因已整合至烟草基因组中,并发生了转录。对T_0代转基因烟草以-1℃处理48h,转基因烟草的相对电导率和表型明显优于野生型烟草。室温恢复试验验证,转基因烟草可存活并恢复生长,而野生型烟草受到了不可逆的低温冻害。研究证明,转化后携带昆虫抗冻蛋白基因的烟草比野生型烟草具有明显的抗寒能力,该结果为减轻冷敏感经济作物在春季遭受霜害提供了理论依据和应用基础。
英文摘要	Most of crops are susceptible to cold and frost.When temperature decreases to -1℃,ice makes plant cells desiccated and cellular membrane shrunk.Expression of insect antifreeze protein genes with very high thermal hysteresis activity in plants is a possible way for increasing the cold tolerance of cold-sensitive plants.The MPAFP149 gene from Xinjiang desert insect Mi-crodera punctipennis dzhunarica was constructed to the plant expression vector pCAMBIA1302 by sub-cloning,and then the recombined vector pCAMBIA1302-MPAFP149 was transformed into Agrobacterium tumefacines EHA105.Transgenic tobacco was obtained via leaf-disc method by Agrobacterium-mediated transformation.PCR and PCR-Southern analysis showed that the MPAFP149 gene was successfully integrated into the tobacco genome.The result of RT-PCR also verified that MPAFP149 gene was transcripted at mRNA level.The relative conductivity of T_0 generation of transgenic tobacco at -1℃ for 48 h was 28.83%,significantly lower than that of wild tobacco which was 82.91%.Meanwhile,the phenotype of transgenic tobacco was superior to that of wild tobacco,suggesting that transgenic tobacco had better cold tolerance than wild tobacco.Recovering experiment at the room temperature indicated that transgenic tobacco was able to recover from cold stress and regenerate.The results proved that transgenic tobacco carrying MPAFP149 gene was more cold tolerant than wild tobacco,which provided a theoretical and applied foundation for relieving cold damage to cold-sensitive crops in spring.
中文关键词	昆虫抗冻蛋白 ; 植物表达载体 ; 烟草 ; 抗寒性
英文关键词	Antifreeze proteins Plant expression vector Tobacco Cold tolerance
语种	中文
国家	中国
收录类别	CSCD
WOS类目	HORTICULTURE
WOS研究方向	Agriculture
CSCD记录号	CSCD:3226249
来源机构	新疆大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/223250
作者单位	新疆大学生命科学与技术学院, 新疆生物资源基因工程重点实验室, 乌鲁木齐, 新疆 830046, 中国
推荐引用方式 GB/T 7714	王艳,邱立明,谢文娟,等. 昆虫抗冻蛋白基因转化烟草的抗寒性[J]. 新疆大学,2008,34(3):397-402.
APA	王艳.,邱立明.,谢文娟.,黄薇.,叶锋.,...&马纪.(2008).昆虫抗冻蛋白基因转化烟草的抗寒性.,34(3),397-402.
MLA	王艳,et al."昆虫抗冻蛋白基因转化烟草的抗寒性".34.3(2008):397-402.