干旱区生态环境知识资源中心(Arid): 应用16SrDNA-RFLP方法分析宁夏地区稻田土壤细菌的多样性

Arid

	应用16SrDNA-RFLP方法分析宁夏地区稻田土壤细菌的多样性
其他题名	Bacteria diversity in paddy field soil by 16S rDNA-RFLP analysis in Ningxia
	张建萍; 董乃源; 余浩滨; 周勇军; 陆永良; 耿锐梅; 余柳青
来源期刊	生物多样性
ISSN	1005-0094
出版年	2008
卷号	16 期号:6 页码:586-592
中文摘要	水稻是宁夏地区主要粮食作物，水稻种植也具有维持生态系统平衡，防止土地荒漠化等重要的生态功能。而稻田土壤细菌是维持土壤生态功能的基础。但长期以来缺乏对干旱地区稻田土壤细菌多样性的认识。本研究采用非培养技术提取稻田土壤样品总DNA，构建其16S rDNA克隆文库，用PCR-RFLP分析进一步测序后聚类分析细菌群落的多样性。从稻田十样中分离获得了大于23kb的DNA片段。PCRRFLP共得到74种酶切带型，序列分析发现77．3％的克隆序列与环境中未培养细菌的16S rDNA序列有较高的相似性，仅有22．7％的克隆序列与数据库中可培养细菌有较高的相似性，表明宁夏稻区土壤中的多数细菌尚未培养。系统发育研究发现74个序列分属于12个类群，其中变形细菌所占比例最大（37．8％），依次为酸杆菌（16．2％）、放线菌（12．2％）、拟杆菌（10．8％）、绿屈挠菌（10．8％）、浮游霉菌（8．1％），另外有少量厚壁菌门、芽单胞菌门和疣微菌门细菌克隆。在变形细菌的序列中包括alpha、beta、gamma和delta4个类型，比例分别为13．5％、5．4％、12．2％和6．8％。表明宁夏稻区土壤中优势细菌类群为变形杆菌和酸杆菌，且土壤细菌类群具有丰富的多样性。
英文摘要	Rice is one of the most important crops in the Ningxia region of China, and rice planting helps to maintain ecosystem balance and prevent land desertification. Soil microbial diversity provides basic functions for rice field soil ecosystems. To better understand bacterial diversity and community composition in Ningxia paddy soil, the total bacterial DNA was extracted from paddy soil collected from a typical rice field of Ningxia using the culture independent method. A 16S ribosomal DNA （16S rDNA） clone library of soil bacteria was constructed. The 16S rDNA fragments were analyzed by PCR-RFLP. Further sequencing and cluster analysis were conducted to elucidate the bacterial diversity. Over 23 kb DNA fragments were obtained from the paddy soil and 74 MspI restriction endonuclease types were detected by PCR-RFLP analysis. Sequence analysis revealed that 77.3% of clone sequences were similar to those of uncultured bacteria in the environment, while only 22.7% clone sequences were most closely related to those of cultured bacteria in GenBank, suggesting great potential for undeveloped bacterial resources was available in paddy fields. Our phylogenetic analysis found that the sequenced clones fell into 12 major lineages within the domain bacteria. Among them, members of the Proteobacteria were the dominant group, accounting for 37.8%, including alpha-Proteobacteria （13.5%）, gamma-Proteobacteria （12.2%）, delta-Proteobacteria （6.8%） and beta-Proteobacteria （5.4%）, followed by Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi and Planctomycetes division with 16.2%, 12.2%, 10.8%, 10.8%, 8.1%, respectively. Firmicutes, Gemmatimonadetes, and Verrucomicrobia were less well represented. Our study revealed an extensive diversity of soil bacteria in a paddy field in Ningxia.
中文关键词	水稻田 ; 土壤细菌多样性 ; rDNA克隆文库 ; RFLP分析
英文关键词	16S paddy rice field bacterial diversity 16S rDNA RFLP analysis
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Agriculture
WOS研究方向	Agriculture
CSCD记录号	CSCD:3453987
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/222729
作者单位	张建萍, 中国水稻研究所, 水稻生物学国家重点实验室, 杭州, 浙江 310006, 中国.; 董乃源, 中国水稻研究所, 水稻生物学国家重点实验室, 杭州, 浙江 310006, 中国.; 余浩滨, 中国水稻研究所, 水稻生物学国家重点实验室, 杭州, 浙江 310006, 中国.; 周勇军, 中国水稻研究所, 水稻生物学国家重点实验室, 杭州, 浙江 310006, 中国.; 陆永良, 中国水稻研究所, 水稻生物学国家重点实验室, 杭州, 浙江 310006, 中国.; 耿锐梅, 中国水稻研究所, 水稻生物学国家重点实验室, 杭州, 浙江 310006, 中国.; 余柳青, 中国水稻研究所, 水稻生物学国家重点实验室, 杭州, 浙江 310006, 中国.
推荐引用方式 GB/T 7714	张建萍,董乃源,余浩滨,等. 应用16SrDNA-RFLP方法分析宁夏地区稻田土壤细菌的多样性[J],2008,16(6):586-592.
APA	张建萍.,董乃源.,余浩滨.,周勇军.,陆永良.,...&余柳青.(2008).应用16SrDNA-RFLP方法分析宁夏地区稻田土壤细菌的多样性.生物多样性,16(6),586-592.
MLA	张建萍,et al."应用16SrDNA-RFLP方法分析宁夏地区稻田土壤细菌的多样性".生物多样性 16.6(2008):586-592.