干旱区生态环境知识资源中心(Arid): 高效液相色谱-串联质谱法测定水产品中硝基呋喃代谢物

Arid

	高效液相色谱-串联质谱法测定水产品中硝基呋喃代谢物
其他题名	Determination of Nitrofuran Metabolites in Aquatic Products by HPLC-MS/MS
	李佐卿; 俞雪钧; 倪梅林; 章再婷; 谢东华; 湛嘉; 殷居易
ISSN	1001-4020
出版年	2008
卷号	44 期号:11 页码:1028-1030,1034
中文摘要	用高效液相色谱-串联质谱法测定水产品中硝基呋喃代谢物的含量.样品经稀盐酸水解并用2-硝基苯甲醛(2-NBA)衍生,调节其酸度至pH 7.0～7.5,离心,将上清液过Oasis HLB(6 mL)小柱后,以乙酸乙酯为洗脱剂,将洗脱液于40℃在氮气流中挥干,并用乙腈与乙酸(1+99)溶液以体积比3比7混合的溶液溶解.用氘代试剂内标法定量.硝基呋喃代谢物标准的质量浓度在4.5mug·L-1以内呈线性,回收率在80.2%～98.4%之间,相对标准偏差(n=8)在3.75%～8.12%之间,测定限(10S/N)为0.25ug·kg-1.
英文摘要	In this paper,a method of HPLC-MS-MS for the determination of nitrofuran metabolites in aquatic products was proposed.The sample was hydrolyzed with dilute hydrochloric acid then derivatized with 2- nitrobenzene formaldehyde(2-NBA).The derivatized solution was adjusted to pH 7.0- 7.5 then separated by centrifugation.The supernatant was introduced into the Oasis HLB microcolumn(6 mL)and eluted with ethyl acetate.The eluate was dried in a nitrogen flow at 40℃,and dissolved with a mixture of acetonitrile and acetic acid (1+99)which were mixed in the ratio of 3 to 7 by volume.The metabolites were determined by HPLC-MS/MS using deuterium reagent as internal standard.Linearity of the calibration curves was in the range within 4.5mug·L-1.Determination limit(10S/N)of the method was 0.25mug·kg-1.Recovery was ranged from 80.2% to 98.4%,and RSD’s(n=8)from 3.75% to 8.12%.
中文关键词	高效液相色谱-串联质谱法 ; 水产品 ; 硝基呋喃代谢物
英文关键词	HPLC-MS/MS Aquatic products Nitrofuran metabolites
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3484785
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/222557
作者单位	宁波出入境检验检疫局, 宁波, 浙江 315012, 中国
推荐引用方式 GB/T 7714	李佐卿,俞雪钧,倪梅林,等. 高效液相色谱-串联质谱法测定水产品中硝基呋喃代谢物[J],2008,44(11):1028-1030,1034.
APA	李佐卿.,俞雪钧.,倪梅林.,章再婷.,谢东华.,...&殷居易.(2008).高效液相色谱-串联质谱法测定水产品中硝基呋喃代谢物.,44(11),1028-1030,1034.
MLA	李佐卿,et al."高效液相色谱-串联质谱法测定水产品中硝基呋喃代谢物".44.11(2008):1028-1030,1034.