干旱区生态环境知识资源中心(Arid): 超高效液相色谱-串联质谱测定动物源性食品和尿液中4种beta-受体激动剂残留

Arid

	超高效液相色谱-串联质谱测定动物源性食品和尿液中4种beta-受体激动剂残留
其他题名	Determination of Four Beta-Agonists Residues in Products of Animal Origin and Urine by Ultra Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry
	王凤美; 张鸿伟; 庞士平; 汤志旭; 牛增元; 罗忻
ISSN	0253-3820
出版年	2008
卷号	36 期号:12 页码:1629-1635
中文摘要	建立了动物源性食品和尿液中4种beta-受体激动剂克伦特罗、莱克多巴胺、沙丁胺醇和特布他林的超高效液相色谱-串联质谱测定方法。样品均质后,加入乙酸铵水溶液,采用beta-葡萄糖醛酸酶/芳基硫酸酯酶酶解处理,过滤后,用OasisMCX固相萃取柱净化,采用UPLC-MS/MS多反应监测(MRM)模式检测,内标法定量。4种beta-受体激动剂的检出限均为0.1mug/kg,定量下限为0.5mug/kg;在0.5、0.75和1.0mug/kg 3个浓度添加水平,总体平均回收率为86.7%～114.3%,总体相对标准偏差均在10%以内。本方法分析速度快,灵敏度高,重现性好,各项技术指标均满足国内外相关法规要求,可用于各种动物源性食品及尿液中4种beta-受体激动剂残留的快速检测。
英文摘要	A method based on ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) has been developed for the determination of beta-agonists residues including clenbuterol,ractopamine,salbutamol and terbutalin in products of animal origin and unine.The analytes were extracted with NH4Ac solution from homogenized samples and hydrolysised with beta-glucuronidase overnight at 37 ℃.Then the extract was filtered and the filtrate was cleaned by Oasis MCX solid phase extraction cartridge.Quantification of the four analytes was achieved by UPLC-MS/MS with multiple reaction monitoring(MRM) using internal standard method.Detection limits and quantification limits of UPLC-MS/MS for the four analytes were all 0.1 mg/kg and 0.5 mg/kg.The overall recovery varies from 86.7% to 114.3% at the spiked level of 0.5 mg/kg,0.75 mg/kg,1.0 mg/kg,and the relative standard deviation(RSD) of the method was less than 10%.The method is reliable,sensitive and reproducible,its performance can meet the requirements of the domestic and international legislation.The method adapts to the determination of beta-agonists residues in different products of animal origin and urine.
中文关键词	beta-受体激动剂 ; 动物源性食品 ; 超高效液相色谱-串联质谱
英文关键词	beta-Agonists products of animal origin ultra performance liquid chromatography-tandem mass spectrometry
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY MULTIDISCIPLINARY
WOS研究方向	Chemistry
CSCD记录号	CSCD:3434621
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/222212
作者单位	山东出入境检验检疫局检验检疫技术中心, 青岛, 山东 266002, 中国
推荐引用方式 GB/T 7714	王凤美,张鸿伟,庞士平,等. 超高效液相色谱-串联质谱测定动物源性食品和尿液中4种beta-受体激动剂残留[J],2008,36(12):1629-1635.
APA	王凤美,张鸿伟,庞士平,汤志旭,牛增元,&罗忻.(2008).超高效液相色谱-串联质谱测定动物源性食品和尿液中4种beta-受体激动剂残留.,36(12),1629-1635.
MLA	王凤美,et al."超高效液相色谱-串联质谱测定动物源性食品和尿液中4种beta-受体激动剂残留".36.12(2008):1629-1635.