干旱区生态环境知识资源中心(Arid): 动物组织中8种同化性激素及代谢物残留量的检测方法研究

Arid

	动物组织中8种同化性激素及代谢物残留量的检测方法研究
其他题名	Determination of 8 Anabolic Hormones and Metabolites Residues in Animal Tissue by Liquid Chromatography-Tandem Mass Spectrometry Method
	许泓; 林安清; 古珑; 吴延晖; 何佳; 张曼; 张骏; 唐丹舟
ISSN	1004-4957
出版年	2008
卷号	27 期号:12 页码:1278-1282
中文摘要	采用液相色谱-串联质谱(LC-MS/MS)测定了动物组织中的去甲雄三烯醇酮(TRE)、甲基睾丸酮(MTS)、丙酸睾酮(PTS)、苯丙酸诺龙(PNT)、醋酸甲羟孕酮(MEDA)、苯甲酸雌二醇(BES)、己烯雌酚(DES)及代谢物双烯雌酚(DEN)、玉米赤霉醇(ZER)及代谢物玉米赤霉酮(ZEAR)、玉米赤霉烯醇(ZEL)、玉米赤霉烯酮(ZEN).动物组织经均质后,用叔丁基甲基醚和乙酸盐缓冲液加酶解剂分别提取试样中的残留激素及代谢物,经硅胶柱和Oasis柱净化,应用LC-MS/MS大气压化学电离正方式(APCI +)、负方式(APCI-)和电喷雾电离正方式(ESI +),以多反应离子监测(MRM)方式进行检测,方法检测能力(CCbeta)为0.028 ～0.273 ng·g-1.选用ZER-d4、DES-d8和MEDA-d3的同位素标记物作内标,内标法定量.在0.5 ～10 ng·g-1范围内回收率为51% ～120%.
英文摘要	A liquid chromatography -tandem mass spectrometry(LC -MS/MS) method was developed for the determination of 8 anabolic hormones including trenbolone ( TRE ) ,methyl testosterone ( MTS ) testosterone propionate ( PTS ) ,nanotrolone phenypropionate ( PNT ) ,medroxyprogesterone mcetate (MDEA),estradiol benzoate ( BES) ,diethylstilbestrol ( DES ) and metabolites dienoestrol ( DEN ) ,zearalanol ( ZER ) and metabolites,including zearalanone ( ZEAR ) ,zearalenol ( ZEL ) ,zearalenone (ZEN) in animal tissues. Animal tissues were firstly homogenized and then extracted by methyl tert-butyl ether(MTBE) and beta-glueuronidase. The sample was cleaned up by solid-phase extraction (SPE) with a silica gel cartridge and an Oasis cartridge. The MS system was operated using atmospheric pres-sure chemical ionization( APCI) in positive and negative mode,and electrospray ionization( ESI) in positive mode. The product ion fragments were monitored by multiple reaction monitoring ( MRM) mode. Detection capabilities ( CCbeta) related to the transition products of lowest abundance were 0.028 -0. 273 ng·g-1. The ZER-d4,DES-d8 and MEDA-d3 were used as internal standards. The recoveries were 51%-120% in the spiking range of 0. 5-10 ng·g-1.
中文关键词	液相色谱-串联质谱 ; 动物组织 ; 残留 ; 检测 ; 同化性激素 ; 代谢物
英文关键词	liquid chromatography-tandem mass spectrometry animal tissue residue determina-tion anabolic hormones metabolites
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3497870
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/222210
作者单位	天津出入境检验检疫局, 天津中国
推荐引用方式 GB/T 7714	许泓,林安清,古珑,等. 动物组织中8种同化性激素及代谢物残留量的检测方法研究[J],2008,27(12):1278-1282.
APA	许泓.,林安清.,古珑.,吴延晖.,何佳.,...&唐丹舟.(2008).动物组织中8种同化性激素及代谢物残留量的检测方法研究.,27(12),1278-1282.
MLA	许泓,et al."动物组织中8种同化性激素及代谢物残留量的检测方法研究".27.12(2008):1278-1282.