干旱区生态环境知识资源中心(Arid): 蜂蜜中14种喹诺酮类药物残留的高效液相色谱-串联质谱测定

Arid

	蜂蜜中14种喹诺酮类药物残留的高效液相色谱-串联质谱测定
其他题名	Simultaneous Determination of 14 Quinolones Residues in Honey by High Performance Liquid Chromatography- Tandem Mass Spectrometry
	曹彦忠 1; 庞国芳 1; 张进杰 1; 石玉秋 1; 李学民 1; 范春林 1; 刘永明 1; 贾光群 1; 刘晓茂 1; 张艳梅 2
ISSN	1004-4957
出版年	2008
卷号	27 期号:11 页码:1141-1146
中文摘要	建立了同时测定蜂蜜中14种喹诺酮类药物残留的高效液相色谱-串联质谱分析方法。蜂蜜中喹诺酮类药物残留用0．05mol／L磷酸盐缓冲溶液（pH：3．0）提取，样液过滤后，经OasisHLB固相萃取柱净化，氢氧化铵-甲醇溶液（体积比1：19）洗脱，蒸干定容后，用反相液相色谱分离，电喷雾正离子模式离子化，用多反应监测方式（MRM）监测，三重四极杆质谱测定。环丙沙星在0．4～100．0mug／kg，嗯喹酸在0．4～50．0mug/kg，恩诺沙星、沙拉沙星、双氟沙星、依诺沙星、诺氟沙星在1．0～100．0mug/kg，氧氟沙星、单诺沙星、氟罗沙星、奥比沙星、麻保沙星在1．0～50．0mug/kg，司帕沙星、氟甲喹在2．0～100．0mug／kg范围内呈线性关系，相关系数r〉0．997，在2．0、5．0、10．0、50．0mug/kg4个添加水平，回收率为66％～111％，相对标准偏差（RSD）为1．3％-13．6％。该方法操作简便，稳定性好，选择性好，灵敏度高，其检出限达1．0mug／kg。
英文摘要	A high performance liquid chromatography- tandem mass spectrometry （HPLC -MS/MS） method was established for simultaneous determination of 14 quinolones residues in honey. The sam- ple were extracted with 0.05 mol/L phosphate buffer solution（ pH = 3.0）. After filtration, the extract was purified by Oasis HLB solid phase extraction column and eluted with methanol - ammonia solution （ 19：1 ,by volume） , and then it was evaporated to dry under nitrogen and redissolved in 1 mL dissolving solution. The solution was separated on a RP-HPLC column and detected by triple-quadrupole MS. The electrospray was operated in the positive ionization mode and the quinolones were identified by using multiple reaction monitoring（MRM） mode. Linear ranges for 14 quinolones were as follows： eiprofloxacin（0.4 - 100.0 mug/kg） ; oxolinic acid（0. 4 - 50.0 mug/kg） ; enrofloxacin, sarafloxacin, difloxaein, enoxacin and norfloxaein（ 1.0 -100.0 mug/kg） ; ofloxaein, danofloxacin, fleroxacin, orbi- floxacin and marbofloxacin（ 1.0 - 50. 0 mug/kg） ; sparfloxacin and flumequin（2.0 - 100. 0 mug/kg）. Correlation coefficients were more than 0. 997. Recoveries at the four spiked levels of 2. 0, 5.0, 10. 0 and 50. 0 mug/kg ranged from 66% - 111% , and relative standard deviations（RSDs） were in the range of 1.3% - 13.6% . The limits of detection for the method were 1.0 mug/kg. Owing the merits of sire-plicity, selectivity and sensitivity, the proposed method has been successfully applied to the determination of quinolones residues in honey.
中文关键词	蜂蜜 ; 喹诺酮类药物 ; 固相萃取 ; 液相色谱-串联质谱法
英文关键词	honey quinolones solid phase extraction liquid chromatography -tandem mass spec- trometry
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3473423
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/222209
作者单位	1.秦皇岛出入境检验检疫局, 秦皇岛, 河北 066002, 中国; 2.河北大学化学与环境科学学院, 保定, 河北 071002, 中国
推荐引用方式 GB/T 7714	曹彦忠,庞国芳,张进杰,等. 蜂蜜中14种喹诺酮类药物残留的高效液相色谱-串联质谱测定[J],2008,27(11):1141-1146.
APA	曹彦忠.,庞国芳.,张进杰.,石玉秋.,李学民.,...&张艳梅.(2008).蜂蜜中14种喹诺酮类药物残留的高效液相色谱-串联质谱测定.,27(11),1141-1146.
MLA	曹彦忠,et al."蜂蜜中14种喹诺酮类药物残留的高效液相色谱-串联质谱测定".27.11(2008):1141-1146.