干旱区生态环境知识资源中心(Arid): 动物组织中卡巴氧和喹乙醇以及相关代谢产物的液相色谱-串联质谱检测方法

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	动物组织中卡巴氧和喹乙醇以及相关代谢产物的液相色谱-串联质谱检测方法
其他题名	Determination of the Residues of Carbadox,Olaquindox and Related Metabolites in Bovine and Porcine Muscle and Liver Tissues by LC-MS/MS
	欧阳姗 1; 庞国芳 2; 谢丽琪 1; 蓝芳 1; 林黎 1; 涂小珂 1
ISSN	1004-4957
出版年	2008
卷号	27 期号:6 页码:590-594
中文摘要	建立了牛、猪肌肉和肝脏组织中卡巴氧(CBX)和喹乙醇(OQX)以及相关代谢产物--脱氧卡巴氧(DCBX)、喹噁啉-2-羧酸(QCA)和3-甲基喹噁啉-2-羧酸(MQCA)残留的LC-MS/MS的检测方法.组织样品中的卡巴氧用乙腈-乙酸乙酯(体积比1 : 1)溶液提取;代谢产物的提取则是经适当处理后的样品加入Protease蛋白酶进行酶解,后采用阴离子交换固相萃取柱Oasis MAX进行净化和富集.分析样品以甲酸溶液(体积分数为 0.1%)-甲醇-乙腈为流动相,经Inertsil ODS-3色谱柱分离,在LC-MS/MS多反应监测模式下进行定性、定量分析,采用正离子扫描.CBX、DCBX、QCA和MQCA的定量下限均为0.5 mug/kg,猪、牛肌肉和肝脏在0.5 ～5.0 mug/kg添加水平的平均回收率在76% ～97%之间,相对标准偏差(n＝10)在2.9% ～16.9%之间.
英文摘要	A liquid chromatography-tandam mass spectrometric(LC-MS/MS) method was developed for the simultaneous identification and determination of residues of carbadox(CBX),olaquindox and related metabolites,desoxycarbadox(DCBX),quinoxalie-2-carboxylic acid(QCA) and 3-methylquinoxaline-2-carboxylic acid(MQCA) in bovine and porcine muscle and liver tissues.CBX was extracted by acetonitrile-ethyl acetate(1∶1,by volume).As for the extraction of DCBX,QCA and MQCA,homogenized liver or muscle tissue was first digested with 0.6%formic acid to deactivate naturally-occurring enzymes.After overnight enzymatic hydrolysis with protease,the tissue extract was acidified,centrifuged and filtered.DCBX,QCA and MQCA were recovered from the tissue extract using an Oasis MAX solid phase extraction(SPE) cartridge.The analytes were separated on an Inertsil ODS-3 LC column,and determined qualitatively and quantitatively under multi-reaction monitoring(MRM) scan type with tandem mass analyzer using positive polarity mode.The limits of quatitation(LOQ) for the four analytes in bovine and porcine muscle and liver tissues were 0.5 mug/kg.At the spiked level in the range of 0.5-5.0 mug/kg,the mean recoveries were in the range of 76%-97%,the relative standard deviation were in the range of 2.9%-16.9%.
中文关键词	卡巴氧 ; 喹乙醇 ; 代谢产物 ; 脱氧卡巴氧 ; 喹噁啉-2-羧酸 ; 3-甲基喹噁啉-2-羧酸 ; 残留分析 ; 液相色谱-质谱
英文关键词	carbadox olaquindo metabolites desoxycarbadox(DCBX) quinoxalie-2-carboxylic acid(QCA) 3-methylquinoxaline-2-carboxylic acid(MQCA) multi-residue analysis LC-MS/MS
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:3312524
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/222207
作者单位	1.深圳出入境检验检疫局, 深圳, 广东 518067, 中国; 2.秦皇岛出入境检验检疫局, 秦皇岛, 河北 066002, 中国
推荐引用方式 GB/T 7714	欧阳姗,庞国芳,谢丽琪,等. 动物组织中卡巴氧和喹乙醇以及相关代谢产物的液相色谱-串联质谱检测方法[J],2008,27(6):590-594.
APA	欧阳姗,庞国芳,谢丽琪,蓝芳,林黎,&涂小珂.(2008).动物组织中卡巴氧和喹乙醇以及相关代谢产物的液相色谱-串联质谱检测方法.,27(6),590-594.
MLA	欧阳姗,et al."动物组织中卡巴氧和喹乙醇以及相关代谢产物的液相色谱-串联质谱检测方法".27.6(2008):590-594.