干旱区生态环境知识资源中心(Arid): 中国西北内陆干旱区大叶白麻野生居群遗传多样性研究

Arid

	中国西北内陆干旱区大叶白麻野生居群遗传多样性研究
其他题名	Genetic Diversity in Wild Populations of Poacynum hendersonii from Inland Arid Regions of Northwest China
	赵金凤 1; 陈敏敏 1; 张卫明 1; 唐泽紫 1; 顾龚平 2; 陆长梅 1
ISSN	0517-6611
出版年	2008
卷号	36 期号:33 页码:14445-14447,14458
中文摘要	[目的]研究大叶白麻野生居群的遗传多样性。[方法]采用RAPD技术对分布于新疆、甘肃和青海的5个大叶白麻野生居群的遗传多样性水平进行检测。[结果]经筛选的20条引物共扩增出165条清晰、重复性好的DNA条带,其中多态性条带为110条,多态位点百分率(PPB)为66.67%。居群间Nei′s多样性指数为0.2205,Shannons信息指数为0.3047,遗传分化系数(Gst)为0.9082。大叶白麻具有较高的遗传多样性水平,遗传分化主要发生在居群间。聚类分析显示居群间的相似度与分布区的地理和气候特征相关。[结论]在利用大叶白麻资源的同时,应根据各居群的品质和遗传特征,对这些居群尤其是青海居群加以保护.
英文摘要	[Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.[Method] Random amplified polymorphic DNA(RAPD) technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai province.[Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei′s gene diversity index(H),Shannon’s information index(I) and genetic differentiation coefficient(Gst) were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.[Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population.
中文关键词	大叶白麻 ; 野生居群 ; 遗传多样性
英文关键词	RAPD Poacynum hendersonii Wild population Genetic diversity RAPD
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRONOMY
WOS研究方向	Agriculture
CSCD记录号	CSCD:3441311
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/221988
作者单位	1.南京师范大学生命科学学院, 南京, 江苏 210046, 中国; 2.南京野生植物综合利用研究院, 南京, 江苏 210042, 中国
推荐引用方式 GB/T 7714	赵金凤,陈敏敏,张卫明,等. 中国西北内陆干旱区大叶白麻野生居群遗传多样性研究[J],2008,36(33):14445-14447,14458.
APA	赵金凤,陈敏敏,张卫明,唐泽紫,顾龚平,&陆长梅.(2008).中国西北内陆干旱区大叶白麻野生居群遗传多样性研究.,36(33),14445-14447,14458.
MLA	赵金凤,et al."中国西北内陆干旱区大叶白麻野生居群遗传多样性研究".36.33(2008):14445-14447,14458.