干旱区生态环境知识资源中心(Arid): HPLC法快速测定饲料中硝基咪唑类药物的研究

Arid

	HPLC法快速测定饲料中硝基咪唑类药物的研究
其他题名	Rapid Determination of Nitroimidazole Drugs in Feeds by HPLC Method
	程林丽; 张素霞; 沈建忠; 王战辉; 闻人乐群
ISSN	0517-6611
出版年	2008
卷号	36 期号:5 页码:1893-1894
中文摘要	[目的[为建立一种检测饲料中硝基咪唑类药物的HPLC方法。[方法]提取饲料样品中的甲硝唑、二甲硝唑、罗硝唑和替硝唑，用Oasis MCX小柱进行净化后，以甲醇-水溶液为流动相，梯度洗脱，紫外检测波长为320mm，进行HPLC测定。[结果]甲硝唑、罗硝唑、二甲硝唑和替硝唑的检测限分别为15.7、25.3、27.3和54.9mug/kg。在0.l～10.0mug／ml浓度范围内，4种硝基咪唑类药物色谱峰面积与浓度呈线性相关，相关系数为0.993～0.999，符合对饲料中抗生素分析的要求。4种硝基咪唑类药物平均回收率为82.5％～99.7％，符合对饲料中药物添加剂分析的准确度要求，变异系数为3.5％～14.0％，符合残留分析对精密度要求。[结论]该方法操作简单、结果准确，可用于测定饲料中硝基咪唑类药物的含量。
英文摘要	[Objective] The research aimed to establish a kind of HPLC method for detecting nitroimidazole drugs in feeds. [Method] Metronidazole, dimetfidazole, ronidazole and tinidazole were extracted from feed samples. They were purified by using Oasis MCX columella and determined with HPLC at the ultraviolet detection wavelength of 320 nm by taking methanol-water solution as mobile phase and using the technology of gradient elution. [Result] The detection limit for metronidazole, ronidazole, dimetridazole and tinidazole were 15.7, 25.3, 27.3 and 54.9 mug/kg respectively. In the concentration range of 0.1～10.0mug/ml, the chromatographic peak area of 4 kinds of nitroimidazole drugs showed linear correlation to their concentrations, with correlation coefficients of 0,993～0.999, which was accordant with the demands of antibiotics analysis in feeds, The average recovery of 4 kinds of nitroimidazole drugs were 82.5 %～99.7 % ,which was accordant with the accuracy requirement of analyzing drug additives in feeds. Their variation coefficients were 3.5 %～14.0 %, which was accordant with the precision requirement in residue analysis. [Conclusion] The method was simple to operate, with accurate results, which could be used for determining the contents of nitroimidazoles in feeds.
中文关键词	甲硝唑 ; 二甲硝唑 ; 罗硝唑 ; 替硝唑 ; 饲料 ; 高效液相色谱法
英文关键词	Metronidazole Diruetridazole Ronidazole Tinidazole Feed High-perforruance liquid chromatography
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:3234301
来源机构	中国农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/221946
作者单位	中国农业大学动物医学院, 北京 100094, 中国
推荐引用方式 GB/T 7714	程林丽,张素霞,沈建忠,等. HPLC法快速测定饲料中硝基咪唑类药物的研究[J]. 中国农业大学,2008,36(5):1893-1894.
APA	程林丽,张素霞,沈建忠,王战辉,&闻人乐群.(2008).HPLC法快速测定饲料中硝基咪唑类药物的研究.,36(5),1893-1894.
MLA	程林丽,et al."HPLC法快速测定饲料中硝基咪唑类药物的研究".36.5(2008):1893-1894.