干旱区生态环境知识资源中心(Arid): 基于线粒体细胞色素b基因的中国大沙鼠系统地理格局

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	基于线粒体细胞色素b基因的中国大沙鼠系统地理格局
其他题名	Phylogeographic patterns of the great gerbil Rhombomys opimus in China based on mitochondrial cytochrome b gene sequence variations
	宁恕龙 1; 周立志 1; 张保卫 1; 赵天飙 2; 邹桂祎 1
ISSN	0001-7302
出版年	2007
卷号	53 期号:4 页码:630-640
中文摘要	通过内蒙、新疆、甘肃的41个大沙鼠样品和1个伊朗撒拉克大沙鼠的mtDNA Cyt b基因全序列的遗传分析,对我国大沙鼠（Rhombomys opimus）的分子系统地理学进行了初步探讨.结果表明,我国41个大沙鼠样品的Cyt b基因包含了50个核苷酸变异位点（占全序列的4.39%）,其中转换48个,颠换2个,共定义23个单倍型.在四个地理种群中,内蒙古中部半荒漠区和阿拉善荒漠区的单倍型多样性最高,甘新荒漠区的单倍型多样性最低;北疆荒漠区的核苷酸多样性最高,内蒙古中部半荒漠区的核苷酸多样性最低.分子变异分析（AMOVA）表明,种群间的遗传变异占51.68%,种群内的遗传变异占48.32%.FST统计结果表明,除内蒙古中部半荒漠区与阿拉善荒漠区地理种群之间差异显著外（P＜0.05）,其它地理种群间的差异均极显著（P＜0.01）.基于单倍型的系统树显示,42只大沙鼠形成三支.其中,伊朗撒拉克地区大沙鼠和中国地区大沙鼠之间的亲缘关系比中国两支大沙鼠之间的亲缘关系远;分析表明,中国分布的大沙鼠两支之间分歧时间估计在0.093 Ma前.嵌套支分析表明,大沙鼠历史种群曾发生过异域片段化、受阻碍基因流和持续种群扩张事件.种群扩张分析提示大沙鼠在0.0119 Ma前曾经历过一次种群扩张事件,种群可能受到末次冰期波动的影响.
英文摘要	To address the phylogeographic patterns of great gerbils(Rhombomys opimus)in the arid regions of China,we examined DNA sequence variations in the mitochondrial cytochrome b gene of the 41 individuals from Inner Mongolia,Xinjiang and Gansu,and one from Sarakhs,Iran in GenBank.We detected 50 nucleotide sites were variable(4.39% in the full sequences),including 48 transitions and 2 transversions among the 1 140 base pairs(bp)of the mitochondrial cytochrome b gene.A total of 23 haplotypes were detected.Among the four populations,the haplotype diversity was highest in the semi-desert of central Inner Mongolia(CIM)and Alxan desert(ALX),and lowest in Xinjiang-Gansu desert(XG).The nucleotide diversity was highest in North Xinjiang desert(NX),and lowest in the central Inner Mongolia(CIM).Analysis of molecular variance(AMOVA)revealed that genetic variation within the populations was 48.32%,however among the populations was 51.68%.F_ ST analysis showed that the differentiation was significant(P<0.05)between the populations in the semi-desert of central Inner Mongolia(CIM)and Alxan desert(ALX),and was extremely significant(P<0.01)among other populations.A phylogenetic tree of haplotypes disclosed three lineages of the great gerbil.Two main lineages comprised the individuals from China,in which the divergence time was more than 93 000 years ago.The third lineage,which was the basal one,just was composed of one individual from Iran.Nested clade analysis(NCA)indicated several population events were existed in different hierachical clades,including allopatric fragmentation,restricted gene flow and continuous population expansion.Population demography analysis indicated that great gerbil experienced strong expansion during the past 11 900 years,which indicated that climatic oscillations in the last ice age might have obvious effect on great gerbil populations.
中文关键词	大沙鼠 ; 细胞色素b基因 ; 线粒体DNA ; 遗传结构 ; 系统地理学 ; 嵌套支分析
英文关键词	Great gerbil Rhombomys opimus Cytochrome bgene mtDNA Genetic variation Phylogeography Nested clade analysis
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ZOOLOGY
WOS研究方向	Zoology
CSCD记录号	CSCD:2950698
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/220854
作者单位	1.安徽大学生命科学学院, 安徽省生态工程与生物技术重点实验室, 合肥, 安徽 230039, 中国; 2.内蒙古地方病防治研究中心, 呼和浩特, 内蒙古 010020, 中国
推荐引用方式 GB/T 7714	宁恕龙,周立志,张保卫,等. 基于线粒体细胞色素b基因的中国大沙鼠系统地理格局[J],2007,53(4):630-640.
APA	宁恕龙,周立志,张保卫,赵天飙,&邹桂祎.(2007).基于线粒体细胞色素b基因的中国大沙鼠系统地理格局.,53(4),630-640.
MLA	宁恕龙,et al."基于线粒体细胞色素b基因的中国大沙鼠系统地理格局".53.4(2007):630-640.