干旱区生态环境知识资源中心(Arid): 鞣制皮张DNA的提取

Arid

	鞣制皮张DNA的提取
其他题名	DNA Isolation from Tanned Animal Skins
	贾学渊; 白素英; 徐艳春; 张伟
ISSN	1000-5382
出版年	2007
卷号	35 期号:11 页码:66-69
中文摘要	采用5种方法从鞣制的野猫(Felis silvestris)皮和漠猫(F. bieti)皮中提取DNA,用Cytb基因的通用引物进行PCR扩增,并对扩增结果进行测序,以检验提取效果.结果显示,鞣制皮张中的DNA含量少,高度降解,且含有大量的酶抑制物.用酚-氯仿法可以得到DNA,但需要采用DNA IQTM系统(Promega)的磁珠纯化策略进一步彻底纯化才能达到最佳扩增效果.此外,PCR引物设计时应尽量缩短扩增片段长度以便提高扩增成功率.
英文摘要	Tanned skins are valuable materials for molecular genetic study of wildlife due to their abundance in species and number,multiple sources and long preserving capacity.However,tanned skins have not been widely used for that purpose because of a lot of technical restrictions in DNA isolation.Five methods were tested to isolate DNA from tanned skins of wild cat(Felis silvestris)and Chinese desert cat(F.bieti).In order to test the effectiveness of these methods,three universal primer pairs of Cytb gene were used for PCR amplification and PCR products were directly sequenced.Results indicate that tanned skins still withhold a tinny amount of but highly degraded DNA together with considerable enzyme inhibitors.DNA can be effectively isolated by using conventional phenol-chloroform method,but requires thorough purification using the means of magnetic bead strategy provided by DNA IQTM system(Promega).In addition,the length of amplicons should be reduced to fit the fragment size of isolated DNA template when primers are designed.
中文关键词	鞣制皮张 ; 提取
英文关键词	DNA Tanned skin DNA Isolation
语种	中文
国家	中国
收录类别	CSCD
WOS类目	BIOCHEMISTRY MOLECULAR BIOLOGY
WOS研究方向	Biochemistry & Molecular Biology
CSCD记录号	CSCD:3003099
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/220850
作者单位	东北林业大学, 哈尔滨, 黑龙江 150040, 中国
推荐引用方式 GB/T 7714	贾学渊,白素英,徐艳春,等. 鞣制皮张DNA的提取[J],2007,35(11):66-69.
APA	贾学渊,白素英,徐艳春,&张伟.(2007).鞣制皮张DNA的提取.,35(11),66-69.
MLA	贾学渊,et al."鞣制皮张DNA的提取".35.11(2007):66-69.