干旱区生态环境知识资源中心(Arid): 梭梭胆碱单氧化物酶基因（CMO）的cDNA克隆

Arid

	梭梭胆碱单氧化物酶基因（CMO）的cDNA克隆
其他题名	Study on the cDNA Isolation in the Gene of Choline Monooxygenase （CMO） Gene from Slaxyon mmadenron bunge
	周春娥 1; 耿飒 1; 齐力旺 2
ISSN	0517-6611
出版年	2007
卷号	35 期号:9 页码:2558-2559
中文摘要	甜菜碱被认为是在细胞中起着无毒渗透保护作用的细胞相溶性物质，广泛存在于植物、动物细胞中，高等植物中的甜菜碱生物合成是以胆碱为底物催化合成，即：胆碱甜菜碱醛甜菜碱，其中第一步是甜菜碱合成的限速反应，由胆碱单氧化物酶（CMO）催化。以梭梭为材料，提取总RNA，用RT-PER法合成梭梭CMO的cDNA，约为821bp，测序结果表明，该克隆包含一个完整的开放读码框，编码一个由262个氨基酸构成的多肽，通过BLAST进行同源性比较，发现此基因与菠菜和山菠菜中同源基因CMO的同源性为75％和82％，证明此基因为梭梭的CMO同源基因。
英文摘要	It is known that the betaine is a kind of non-poisonous osmotic solute,which is one of compound substance and is existed extensively in cells of plant and animal.The betaine is catalyzed on choline in plants.That is cholinebetaine aldehydebetaine.Between these two steps,the first step is the most important one that is up to the speed of catalyzed reaction.In this research the Slaxyon mmodenron Bunge was used as material that was taken from TengGeLi Desert in Gansu and the leave of Slaxyon mmodenron Bunge were collected into liquid nitrogen;and then we extracted total RNA from plant leave and counter-transcripted total RNA into complement DNA by reverse transcriptase-polymerase chain reaction(RT-PCR).Based on this,it was followed that choline monooxygenase(CMO) gene correlated with drought resistance were isolated from Slaxyon mmodenron Bung,successfully.According to sequencing result and the compare of homology in GenBank,it was showed that the length of the CMO gene was 821 bp,and there were 75% homology with S.oleracea and 82 % homology with atriplex hortensix.
中文关键词	梭梭 ; 甜菜碱 ; 胆碱单氧化物酶 ; 干旱胁迫
英文关键词	Slaxyon mmodenron bunge Betaine Chollne-monooxygenase（CMO） Drought stress
语种	中文
国家	中国
收录类别	CSCD
WOS类目	GENETICS HEREDITY
WOS研究方向	Genetics & Heredity
CSCD记录号	CSCD:2865027
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/220627
作者单位	1.河南师范大学生命科学学院, 新乡, 河南 453007, 中国; 2.中国林科院林业研究所细胞生物学实验室, 北京 100091, 中国
推荐引用方式 GB/T 7714	周春娥,耿飒,齐力旺. 梭梭胆碱单氧化物酶基因（CMO）的cDNA克隆[J],2007,35(9):2558-2559.
APA	周春娥,耿飒,&齐力旺.(2007).梭梭胆碱单氧化物酶基因（CMO）的cDNA克隆.,35(9),2558-2559.
MLA	周春娥,et al."梭梭胆碱单氧化物酶基因（CMO）的cDNA克隆".35.9(2007):2558-2559.