干旱区生态环境知识资源中心(Arid): TaMyb2-Ⅱ基因在普通小麦(Triticum aestivum L.)及其近缘种中的单核苷酸多态性分析

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	TaMyb2-Ⅱ基因在普通小麦(Triticum aestivum L.)及其近缘种中的单核苷酸多态性分析
其他题名	Single Nucleotide Polymorphism of TaMyb2-Ⅱ Gene in Common Wheat (Triticum aestivum L.) and Its Relatives
	王爱萍 1; 毛新国 2; 景蕊莲 2; 昌小平 2; 杨武德 1
ISSN	0496-3490
出版年	2006
卷号	32 期号:12 页码:1809-1816
中文摘要	以39份抗旱性不同的普通小麦、5份A基因组材料、4份拟斯卑尔脱山羊草(Aegilops speltoides)、6份粗山羊草(A.tauschii)和2份四倍体小麦为材料,分析TaMyb2基因的核苷酸序列长度多态性和单核苷酸多态性,及其与抗旱性的关系.结果发现,TaMyb2在A基因组材料中无目标片段扩增,在其他材料中共检测到Ⅰ、Ⅱ、Ⅲ 3种类型序列.经详细分析,TaMyb2-Ⅱ序列长1 606 bp,在供试材料77 088 bp的核苷酸序列中检测到34个单核苷酸变异,其中26个SNP,8个InDel,二者出现的频率分别为1/2 965 bp和1/9 636 bp,编码区pi值(0.000 55)小于非编码区的pi值(0.001 85), 说明编码区的遗传变异小于非编码区的遗传变异.从SNP水平上分析,发现普通小麦与其D基因组供体种粗山羊草及四倍体小麦的亲缘关系较近,与B基因组供体种拟斯卑尔脱山羊草的亲缘关系较远.48份材料的TaMyb2-Ⅱ序列共分为18个单倍型(haplotype),其中haplotype 2、3、5、6、8、9均为旱地栽培的普通小麦品种,说明普通小麦TaMyb2-Ⅱ的这几个haplotype结构可能与抗旱性有关.
英文摘要	Drought is one of the major elements affecting the yield of wheat, one of the most important crops in arid and semi-arid areas. Mining pivotal drought-resistant genes and probing their polymorphisms is a feasible approach to decipher crops’ drought resistance. The genes involved in drought resistance are classified into two classifications, functional genes and regulatory genes, based on their functions responding to water deficient. Much attention has been paid to regulatory genes because of their crucial roles in drought resistance. AtMyb2 regulates the expression of dehydration-responsive genes rd22 and AtADHl in plants. Recent studies indicate that TaMybl is involved in response to water stress in common wheat, but whether its polymorphisms associated with drought resistance is still unclear. To probe the polymorphisms of TaMyb2 gene and uncover their functions in drought resistance, a total of fifty-six accessions, including thirty-nine common wheat cultivate with different drought resistance, five species with A genome including three T. urartu, one T. morwcoccum and one T. boeoticum, four A. speltoides, six Ae. tauschii and two tetraploid wheat species, were selected to perform the research. The PCR amplification showed that TaMyb2 was detectable in all accessions except A genome species. Based on the genomic sequences, TaMyb2 was classified into 3 types named asTaMyb2-I, TaMyb2-II and TaMyb2-III, respectively. The full-length sequence of TaMyb2-1 geneomic DNA was 1 606 bp. Twenty-six SIN Pa (single nucleotide polymoiphisra) and eight InDels (insertion-deletion) were identified in 77 088 bp tuieleotide acid sequence of 48 accessions, and the frequencies of SNP and InDel were 1/2 965 bp and I /9 636 lip, respectively. The frequency of SNP in coding region (pi = 0.00055) of TaMyb2-II was lower than that in noneoding region (TT = 0.00185), the ratio of Ka/Ks was 0.32 which seemed to suggest TaMyh2-II suffered artificial selection pressure and was very conservative. Phylogenetie analysis demonstrated that the relationship between Ac. tuiisrhii and common wheat was much closer than that between Ae. speltoides and common wheat. Eighteen haplolypes were delected in 48 accessions, in which baplotype 2, 3, 5, 6, 8 and 9 were common wheat eultivars with drought resistance, which indicated that these haplotypes might relate to the drought resistance of common wheat.
中文关键词	单核苷酸多态性 ; 普通小麦 ; 近缘种 ; 抗旱性
英文关键词	TaMyb2-Ⅱ TaMyb2-Ⅱ Single nucleotide polymorphism Common wheat Relative Drought resistance
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRONOMY
WOS研究方向	Agriculture
CSCD记录号	CSCD:2622156
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/220612
作者单位	1.山西农业大学农学院, 太谷, 山西 030801, 中国; 2.中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程, 农业部作物种质资源与生物技术重点开放实验室, 北京 100081, 中国
推荐引用方式 GB/T 7714	王爱萍,毛新国,景蕊莲,等. TaMyb2-Ⅱ基因在普通小麦(Triticum aestivum L.)及其近缘种中的单核苷酸多态性分析[J],2006,32(12):1809-1816.
APA	王爱萍,毛新国,景蕊莲,昌小平,&杨武德.(2006).TaMyb2-Ⅱ基因在普通小麦(Triticum aestivum L.)及其近缘种中的单核苷酸多态性分析.,32(12),1809-1816.
MLA	王爱萍,et al."TaMyb2-Ⅱ基因在普通小麦(Triticum aestivum L.)及其近缘种中的单核苷酸多态性分析".32.12(2006):1809-1816.