干旱区生态环境知识资源中心(Arid): 固相萃取结合HPLC-MS测定人血浆中奥美沙坦的药物质量浓度

Arid

	固相萃取结合HPLC-MS测定人血浆中奥美沙坦的药物质量浓度
其他题名	Quantitative Analysis of Olmesartan in Human Plasma by HPLC-MS With Solid-Phase Extraction
	于洋; 侯艳宁; 刘建芳; 王金
ISSN	1001-2494
出版年	2006
卷号	41 期号:20 页码:1592-1593
中文摘要	目的建立人血浆中奥美沙坦质量浓度的HPLC-MS测定法.方法血浆样品用OASIS HLB 3cc固相萃取小柱萃取,在Diamonsil C_(18)(4.6mm*150 mm,5 mum)色谱柱上分离,流动相为甲醇-水(含体积分数为0.2%醋酸)(70:30),流速0.5 mL·min~(-1).LC-ESI/MS选择离子检测,正离子模式,用于定量分析的离子分别为m/z 447.2(奥美沙坦),m/z 515.3(内标,替米沙坦).结果奥美沙坦血药质量浓度在1～3 000 mug·L~(-1)内的线性关系良好(r=0.999 9),血浆中低、中、高3种质量浓度(3,100,2 400mug·L~(-1))的相对回收率在97.6%～100.9%之间,日内RSD≤5.2%,日间RSD≤13.3%.结论本法操作简便、准确、灵敏,适用于临床药动学研究.
英文摘要	OBJECTIVE To develop a sensitive and specific HPLC-MS method for the quantitative analysis of omlesartan in human plasma.METHODS The plasma sample was extracted by Waters OASIS HLB 3cc solid-phase extraction mini column, then separated on a Dia-monsil C_(18)(4.6 mm * 150 mm, 5 mum)analytical column. The mobile phase consisted of methanol-water(contaning0.2% acetic acid)(70:30) at a flow rate of 0.5 mL·min~(-1). A mass spectrometer equpied with electiiospray ionization source was used as detector and operated in the positive ion mode. In selected ion monitoring(SIM)mode, the ion of m/z 447.2 and m/z 515.3 was used to qualify olmesartan and internal stan-dard(tehnisartan), respectively. RESULTS Chromatograms showed no endogenous peaks with the respective blank human plasma samples. The linearity was obtained in the concentration range of 1～3 000 mug·L~(-1), and the limit of quantitativation was 1 mug·L~(-1).The relative recoveries of low, medium and high concentration were within 97.6%～100.9%. The intra-day and inter-day RSDs were less than 5.2% and 13.3%, respectively. CONCLUSION The method is simple, accurate, sensitive, and suitable for clinical investigation of olmesartan me-doxomil pharmacokinetics.
中文关键词	奥美沙坦 ; 固相萃取 ; 高效液相色谱-质谱联用法 ; 血浆
英文关键词	olmesartan solid-phase extraction HPLC-MS plasma
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PHARMACOLOGY PHARMACY
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:2553500
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/220532
作者单位	白求恩国际和平医院临床药理室, 石家庄, 河北 050082, 中国
推荐引用方式 GB/T 7714	于洋,侯艳宁,刘建芳,等. 固相萃取结合HPLC-MS测定人血浆中奥美沙坦的药物质量浓度[J],2006,41(20):1592-1593.
APA	于洋,侯艳宁,刘建芳,&王金.(2006).固相萃取结合HPLC-MS测定人血浆中奥美沙坦的药物质量浓度.,41(20),1592-1593.
MLA	于洋,et al."固相萃取结合HPLC-MS测定人血浆中奥美沙坦的药物质量浓度".41.20(2006):1592-1593.