干旱区生态环境知识资源中心(Arid): 胶体金免疫层析法检测鼠疫F1抗体技术的应用及效果评价

Arid

	胶体金免疫层析法检测鼠疫F1抗体技术的应用及效果评价
其他题名	Application of and evaluation on Fraction-1 antibodies against Yersinia pestis using gold-immunochromatography assay
	刘合智; 史献明; 白雪薇; 王海峰; 张月芝; 李玉贵; 杜国义; 白小英; 胡乐乐; 杨顺林
ISSN	1000-4955
出版年	2006
卷号	25 期号:6 页码:611-614
中文摘要	目的研究鼠疫胶体金免疫层析法(GICA)快速检测鼠疫F1抗体技术在鼠疫血清学诊断、监测及流行病学调查中的应用及效果评价.方法应用GICA、双抗原夹心酶联免疫吸附试验(DAgS-ELISA)和间接血球凝集试验(IHA)对比检测190份啮齿动物血清和11份鼠疫免疫血清中鼠疫F1抗体.结果 190份动物血清IHA检测结果均为阴性;GICA检测出1份阳性血清,滴度1∶10;DAgS-ELISA检测出3份阳性血清,滴度1∶4(++)2份和1∶16(+)1份.3种方法检测11份鼠疫免疫血清均为9份阳性,但GICA敏感性低于IHA和DAgS-ELISA(GICA与IHA:t=3.257,P＜0.05;GICA与ELISA:t=3.625,P＜0.01;IHA与ELISA:t=1.437,P＞0.05).201份血清检测结果,GICA与IHA总符合率99.50%,与DAgS-ELISA总符合率99.00%,3种方法检测结果差异无统计学意义(chi2=0.5,P＞0.05).结论 GICA检测鼠疫F1抗体敏感特异、简便快速,与DAgS-ELISA同样为鼠疫血清学快速诊断和鼠疫监测中有应用价值的检测技术.
英文摘要	Objective To study application and results of gold-immunochromatography assay (OICA) dipstick for rapid detection of antibodies against Yersinia pestis Fraction-1 (F1) antibodies in plague serodiagnosis, surveillance mid epidemiological investigation. Methods 190 samples of rodent serum specimens from plague focus in Kangbao county and plague surveillance region in Guyuan county, and 11 samples of rabbit serum immunized with Yersinia pestis were detected by means of GIGA lest, double antigens sandwich Enzymelinked immunosorbent assay (DAgS-ELISA) and indirect hemagglutinalion assay (IHA) for antibodies against Yersinia pestis F1. Results The F1 antibodies of 190 samples of rodent serum were negative detected by IHA lest, there was 1 positive portion by G1CA at a titer 1:10; out of 3 positive portions by DAgS-ELISA lest 2 were at a liter 1:4 (++), 1 at 1:16 (+). In 11 samples of rabbit serum going through the three tests, 9 were positive, with the sensitivity of GICA lower than that of IHA and ELISA (GIGA vs IHA: I = 3.257, P< 0.05; GICA vs KL1SA: X~2 = 3.625, P < 0.01; IHA vs ELISA: t= 1.437,P> 0.05). The outcome of GICA was accordant with that of IHA test in a rate of 99.50% and with that of DAgS-KUSA test in a rate of 99.00%. There was no significant discrepancy (x~2 = 0.5, P > 0.05) among the 3 methods. Conclusions The GIGA was sensitive, specific, practicable arid rapid used for detection of VI antibodies; Both the GIGA and DAgS-ELISA are valuable techniques of early diagnosis and surveillance of plague.
中文关键词	胶体金免疫层析 ; 耶尔森菌 ; 鼠疫 ; F1抗 ; 原抗体
英文关键词	Gold-immunochromatography assay Yersinia pestis Fraction-1 antigen Antibodies
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:2657387
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/220389
作者单位	河北省鼠疫防治所检验科, 张家口, 河北 075000, 中国
推荐引用方式 GB/T 7714	刘合智,史献明,白雪薇,等. 胶体金免疫层析法检测鼠疫F1抗体技术的应用及效果评价[J],2006,25(6):611-614.
APA	刘合智.,史献明.,白雪薇.,王海峰.,张月芝.,...&杨顺林.(2006).胶体金免疫层析法检测鼠疫F1抗体技术的应用及效果评价.,25(6),611-614.
MLA	刘合智,et al."胶体金免疫层析法检测鼠疫F1抗体技术的应用及效果评价".25.6(2006):611-614.