干旱区生态环境知识资源中心(Arid): 川芎嗪对大鼠局灶性脑缺血后侧脑室室下区细胞增殖的作用

Arid

	川芎嗪对大鼠局灶性脑缺血后侧脑室室下区细胞增殖的作用
其他题名	Effect of Ligustrazine on Cell Proliferation in Subventricular Zone of Lateral Cerebral Ventricle after Adult Rat Suffering from Focal Cerebral Ischemia
	邱芬; 刘勇; 钱亦华; 赵建军; 田英芳; 祁存芳
ISSN	1672-173X
出版年	2006
卷号	37 期号:5 页码:726-729,780
中文摘要	目的观察川芎嗪对大鼠局灶性脑缺血后侧脑室室下区(SVZ)细胞增殖的作用.方法 SD雄性大鼠随机分为假手术组、缺血模型组和川芎嗪组.线栓法制作大鼠大脑中动脉阻塞模型,川芎嗪组术后2 h腹腔注射川芎嗪(80 mg/kg,1次/d),各组术后4 h腹腔注射5-溴脱氧尿核苷(BrdU,50 mg/kg,1次/d).术后7、14、21 d取材,采用免疫组织化学染色观察SVZ BrdU 阳性细胞数和Doublecortin(DCX)的表达.结果缺血模型组术后7 d时SVZ BrdU 阳性细胞较假手术组明显增加(P＜0.01),并持续至14 d,21 d减少;川芎嗪组14 d SVZ BrdU 阳性细胞达峰值,21 d有所减少,与缺血模型组比较,7、14 d BrdU 阳性细胞均明显增加(P＜0.01).缺血模型组7 d时SVZ有 DCX阳性表达,14 d达最多,21 d表达减少,与假手术组相应时间点比较均明显增加(P＜0.01);川芎嗪组随缺血时间延长SVZ DCX表达明显增强,21 d仍处于高水平,与缺血模型组比较,14、21 d DCX表达明显增强(P＜0.01).结论川芎嗪对成年大鼠局灶性脑缺血诱导的SVZ神经干细胞/祖细胞增殖可能有促进作用.
英文摘要	Objective To explore the effect of ligustrazine on cell proliferation in subventricular zone of lateral cerebral ventricle after middle cerebral artery occluded (MACO) in adult rat. Methods SD male rats were randomly divided into three grpups sham operation group, ischemic model group and Ligustrazine group. The , model of the rrjiddle cerebral artery occlusi6n; was established by placement o( an intraluminal filament at the origin ■ of left MCA.: Ligustrazine was, administered.irjtraperitoneally with a dose of 80 mg/kg daily starting at 2 hours after MCAQ; BrdU (50 mg/kg) was injected.once a day intraperitoneally starting at 4 hours after operation. Number of BrdU-positive cells and expression of doublecortin (DGX) in subventricular zone(SVZ) were measured by immunohistbchemistry on day 7,14,24 aftfer operation. Results Compared with sham operation group, BrdU- positive cells in ischemic model group increased on day 7, reached the peak on day 14, then decreased oh day 21 after operation. On day 7 arid 14, the numbers of BrdU-positive cells in Ligustrazine group were markedly augmented and significantly more than those in ischemic model group CP<0. 01) , but decreased on day 21. The expressions of DCX in SVZ in ischemic model group were enhanced on day 7 with lasting into day 14 > and reduced on day 21, but still higher than those in sham operation group on day 7, 14, and 21, respectively. The expressions of DCX in SVZ in Ligustrazine group increased gradually along with: prolong of ischemia and kept the high level up to day 21 after operation, and were higher than those in ischemic model group on day 14 and 21. Conclusion Our results suggest that Ligustrazine may promote the cells of SVZ, which the adult rats suffer from the focal cerebral ischemia, to go into cell proliferation.
中文关键词	川芎嗪 ; 脑缺血 ; 侧脑室室下区 ; 细胞增殖
英文关键词	Ligustrazine Cerebral ischemia Lateral cerebral ventricle subventricular zone Cell proliferation
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CLINICAL NEUROLOGY
WOS研究方向	Neurosciences & Neurology
CSCD记录号	CSCD:2440308
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/220215
作者单位	西安交通大学医学院,神经科学研究中心人体解剖与组织胚胎学系, 西安, 陕西 710061, 中国
推荐引用方式 GB/T 7714	邱芬,刘勇,钱亦华,等. 川芎嗪对大鼠局灶性脑缺血后侧脑室室下区细胞增殖的作用[J],2006,37(5):726-729,780.
APA	邱芬,刘勇,钱亦华,赵建军,田英芳,&祁存芳.(2006).川芎嗪对大鼠局灶性脑缺血后侧脑室室下区细胞增殖的作用.,37(5),726-729,780.
MLA	邱芬,et al."川芎嗪对大鼠局灶性脑缺血后侧脑室室下区细胞增殖的作用".37.5(2006):726-729,780.