干旱区生态环境知识资源中心(Arid): 蒺藜总皂苷对大鼠急性心肌缺血再灌注损伤的影响

Arid

	蒺藜总皂苷对大鼠急性心肌缺血再灌注损伤的影响
其他题名	Effects of gross saponin from Tribulus terrestris on acute myocardial ischemia/reperfusion injury in rats
	王显刚; 殷惠军; 史大卓
ISSN	0577-7402
出版年	2006
卷号	31 期号:6 页码:531-533
中文摘要	目的观察蒺藜总皂苷（GSTT）对大鼠心肌缺血再灌注损伤（MI／R）的影响。方法可逆性冠脉左前降支结扎缺血45min再灌注2h复制MI／R模型，入选SD雄性大鼠64只，随机分成假手术组、模型组、GSTT大剂量组、GSTT小剂量组，每组16只。HE染色观察心肌病理改变，TTC染色测定心肌梗死面积，检测血清LDH、CPK、MDA含量。结果与模型组比较，GSTT大、小剂量组MI／R心肌病理改变减轻，血清LDH、CPK水平降低（P〈0．01或0．05），且GSTT大剂量组心肌梗死面积缩小（P〈0．05），MDA水平下降（P〈0．05）。结论GSTT对MI／R有保护作用，可改善MI／R心肌病理改变，缩小心肌梗死面积，其机制与减轻氧自由基损伤、稳定心肌细胞膜结构有关。
英文摘要	Objective To investigate the effects of gross saponin from Tribulus terrestris （GSTT） on myocardial ischemia/reperfusion （MI/R） injury. Methods A model of myocardial MI/R injury of the myocardium was reproduced by ligation of left descending artery for 45rain followed by releasing the ligation for 2 hours in rats. 64 SD rats were divided randomly to 4 groups, i.e. sham-operated group, model group, large and low dose of GSTT groups. HE staining was examined to assess myocardial pathological changes. TTC staining was used to determine myocardial infarction area. Serum contents of lactate dehydrogenase （LDH）, ereatine phosphokinase （CPK） and malondialdehyde （MDA） were also determined. Results Both large and low dose of GSTT improved myocardial pathological changes after isehemia/reperfusion. Large dose of GSTT reduced myocardial infarction area （P〈0. 05）. Large and low dose of GSTT could inhibit the release of serum LDH and CPK （P〈0. 01, P〈0. 05）. In addition, large dose of GSTT inhibited the release of MDA （P＇~0. 05）. Conclusion GSTT could protect myocardium from MI/R injury, improve myocardial pathological changes arid reduce myocardial infaretion area. Its mechanism of action was related to ameliorating oxygen free radical injury and stabilizing myocardial cellular membrane.
中文关键词	蒺藜总皂苷 ; 心肌再灌注损伤 ; 心肌梗塞
英文关键词	gross saponins from Tribulus terrestris, myocardial reperfusion injury myocardial infarction
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:2484770
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/219901
作者单位	中国中医研究院西苑医院心内科, 北京 100091, 中国
推荐引用方式 GB/T 7714	王显刚,殷惠军,史大卓. 蒺藜总皂苷对大鼠急性心肌缺血再灌注损伤的影响[J],2006,31(6):531-533.
APA	王显刚,殷惠军,&史大卓.(2006).蒺藜总皂苷对大鼠急性心肌缺血再灌注损伤的影响.,31(6),531-533.
MLA	王显刚,et al."蒺藜总皂苷对大鼠急性心肌缺血再灌注损伤的影响".31.6(2006):531-533.