干旱区生态环境知识资源中心(Arid): 固相萃取反相高效液相色谱法测定人血清中洛美利嗪浓度

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	固相萃取反相高效液相色谱法测定人血清中洛美利嗪浓度
其他题名	Determination of Lomerizine in Human Serum by Reversed Phase High Performance Liquid Chromatography with Solid Phase Extraction
	汤建林; 周世文; 应懿; 王璐; 黄永平
ISSN	0253-3820
出版年	2006
卷号	34 期号:7 页码:995-998
中文摘要	实验建立了固相萃取反相高效液相色谱法测定血清中洛美利嗪的浓度.采用Waters Oasis HLB 萃取小柱对血清样品进行固相萃取,以氟桂利嗪为内标,在Waters高效液相色谱系统上,C8为固定相,乙腈-水（67： 33,含0.2%三乙胺,磷酸调节pH 至6.5）为流动相,流速：1 mL/min;检测波长： 223 nm;柱温：30℃;实验结果显示洛美利嗪和内标物洗脱时间分别为9.9 min和10.8 min,与血中内源性杂质得到良好分离.方法的相对回收率为99.6%～106.1%;绝对回收率为61.8%～86.5%;日内日间变异RSD范围分别为3.6%～5.3%和2.7%～6.9%;线性范围为5～320 mug/L（n=7, r=0.9998）.当S/N＝3时,洛美利嗪最低检出浓度可达5 mug/L.所建方法准确、灵敏,可满足血药浓度监测和药代动力学参数测试的需求.
英文摘要	A reversed phase high performance liquid chromatography with solid-phase extraction for the determination of lomerizine （LOZ） concentration in human serum was established. Using flunarizine （FOZ） as an internal standard （ IS）, solid-phase extraction of human serum with waters oasis HLB column. The Loz and IS were separated on Cs column with acetonitrih-water （67：33, 0. 2% triethylamine, phosphatic acid adjust pH to 6.5） as the mobile phase, flow rate of 1 mL/min, detected at 223 ran, column temperature at 30 ℃. The linear range of calibration curve was within drug concentrations of 5 - 320 mug/L （ n = 7, r = 0. 9998 ）. The lower limit of quantification （ S/N= 3 ） was 5mug/L. Relative recovery was 99.6% - 106. 1%, absolute recovery was 61.8% - 86.5%. Intra-and inter-assay RSD ranged from 3.6% to 5.3% and 2.7% to 6.9%, respectively. The method can be used for detection of lomerizine concentration in human serum and for estimation of pharmacokinetic parameters.
中文关键词	盐酸洛美利嗪 ; 血清 ; 高效液相色谱 ; 固相萃取 ; 药代动力学
英文关键词	Lomerizine serum high performance liquid chromatography solid phase extraction pharmacokinetics
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:2465817
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/219615
作者单位	第三军医大学新桥医院国家药品临床研究基地, 重庆 400037, 中国
推荐引用方式 GB/T 7714	汤建林,周世文,应懿,等. 固相萃取反相高效液相色谱法测定人血清中洛美利嗪浓度[J],2006,34(7):995-998.
APA	汤建林,周世文,应懿,王璐,&黄永平.(2006).固相萃取反相高效液相色谱法测定人血清中洛美利嗪浓度.,34(7),995-998.
MLA	汤建林,et al."固相萃取反相高效液相色谱法测定人血清中洛美利嗪浓度".34.7(2006):995-998.